Suppression of transcription factor early growth response 1 reduces herpes simplex virus 1-induced corneal disease in mice

Hui Wen Yao, Shih Heng Chen, Ching Li, Yuk Ying Tung, Shun Hua Chena

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Herpes simplex virus 1 replication initiates angiogenesis and inflammation in the cornea. This can result in herpetic stromal keratitis (HSK), which is a leading cause of infection-induced corneal blindness. Host cellular factors mediate the progression of HSK, but little is known about these cellular factors and their mechanisms of action. We show here that the expression of the cellular transcription factor early growth response 1 (Egr-1) in HSV-1-infected mouse corneas was enhanced. Enhanced Egr-1 expression aggravated HSK by increasing viral replication and subsequent neovascularization with high levels of potent angiogenic factors, fibroblast growth factor 2, and vascular endothelial growth factor. Furthermore, Egr-1 deficiency due to a targeted disruption of the gene or knockdown of Egr-1 expression topically using a DNA-based enzyme significantly reduced HSK by decreasing both viral replication and the angiogenic response. The present study provides the first evidence that endogenous Egr-1 aggravates HSK and that blocking Egr-1 reduces corneal damage.

Original languageEnglish
Pages (from-to)8568-8580
Number of pages13
JournalJournal of Virology
Volume86
Issue number16
DOIs
Publication statusPublished - 2012 Aug

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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