Surface modification for enhancing antibody binding on polymer-based microfluidic device for enzyme-linked immunosorbent assay

Yunling Bai, Chee Guan Koh, Megan Boreman, Yi Je Juang, I. Ching Tang, L. James Lee, Shang Tian Yang

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

A novel surface treatment method using poly(ethyleneimine) (PEI), an amine-bearing polymer, was developed to enhance antibody binding on the poly(methyl methacrylate) (PMMA) microfluidic immunoassay device. By treating the PMMA surface of the microchannel on the microfluidic device with PEI, 10 times more active antibodies can be bound to the microchannel surface as compared to those without treatment or treated with the small amine-bearing molecule, hexamethylenediamine (HMD). Consequently, PEI surface modification greatly improved the immunoassay performance of the microfluidic device, making it more sensitive and reliable in the detection of IgG. The improvement can be attributed to the spacer effect as well as the functional amine groups provided by the polymeric PEI molecules. Due to the smaller dimensions (140 × 125 μm) of the microchannel, the time required for antibody diffusion and adsorption onto the microchannel surface was reduced to only several minutes, which was 10 times faster than the similar process carried out in 96-weIl plates. The microchip also had a wider detection dynamic range, from 5 to 1000 ng/mL, as compared to that of the microtiter plate (from 2 to 100 ng/mL). With the PEI surface modification, PMMA-based microchips can be effectively used for enzyme linked immunosorbent assays (ELISA) with a similar detection limit, but much less reagent consumption and snorter assay time as compared to the conventional 96-well plate.

Original languageEnglish
Pages (from-to)9458-9467
Number of pages10
JournalLangmuir
Volume22
Issue number22
DOIs
Publication statusPublished - 2006 Oct 24

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Fingerprint Dive into the research topics of 'Surface modification for enhancing antibody binding on polymer-based microfluidic device for enzyme-linked immunosorbent assay'. Together they form a unique fingerprint.

  • Cite this