Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

R. Y. He, K. C. Cho, Nan-Shan Chang, Y. D. Su, S. J. Chen

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1 Citation (Scopus)


A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide the fluorescent images of living cell membranes. The proposed microscope with the helps of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having shorter fluorophore lifetime. In comparison with one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. Combining with the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated the brighter and more contrast fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

Original languageEnglish
Article number71831L
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Publication statusPublished - 2009 Jun 1
EventMultiphoton Microscopy in the Biomedical Sciences IX - San Jose, CA, United States
Duration: 2009 Jan 252009 Jan 27

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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