Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

R. Y. He, K. C. Cho, Nan-Shan Chang, Y. D. Su, S. J. Chen

Research output: Contribution to journalConference article

1 Citation (Scopus)

Abstract

A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide the fluorescent images of living cell membranes. The proposed microscope with the helps of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having shorter fluorophore lifetime. In comparison with one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. Combining with the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated the brighter and more contrast fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

Original languageEnglish
Article number71831L
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7183
DOIs
Publication statusPublished - 2009 Jun 1
EventMultiphoton Microscopy in the Biomedical Sciences IX - San Jose, CA, United States
Duration: 2009 Jan 252009 Jan 27

Fingerprint

Fluorescence microscopy
Cell membranes
Photons
Fluorescence Microscopy
Fluorescence
Cell Membrane
microscopy
Imaging techniques
fluorescence
photons
Microscopes
Plasmons
microscopes
plasmons
Mars Excursion Module
Photobleaching
monkeys
Electromagnetic Fields
Fluorophores
augmentation

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

@article{c0ae0380e17949f1ba0d5aab93b89458,
title = "Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging",
abstract = "A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide the fluorescent images of living cell membranes. The proposed microscope with the helps of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having shorter fluorophore lifetime. In comparison with one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. Combining with the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated the brighter and more contrast fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.",
author = "He, {R. Y.} and Cho, {K. C.} and Nan-Shan Chang and Su, {Y. D.} and Chen, {S. J.}",
year = "2009",
month = "6",
day = "1",
doi = "10.1117/12.809134",
language = "English",
volume = "7183",
journal = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
issn = "1605-7422",
publisher = "SPIE",

}

Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging. / He, R. Y.; Cho, K. C.; Chang, Nan-Shan; Su, Y. D.; Chen, S. J.

In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 7183, 71831L, 01.06.2009.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

AU - He, R. Y.

AU - Cho, K. C.

AU - Chang, Nan-Shan

AU - Su, Y. D.

AU - Chen, S. J.

PY - 2009/6/1

Y1 - 2009/6/1

N2 - A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide the fluorescent images of living cell membranes. The proposed microscope with the helps of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having shorter fluorophore lifetime. In comparison with one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. Combining with the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated the brighter and more contrast fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

AB - A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide the fluorescent images of living cell membranes. The proposed microscope with the helps of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having shorter fluorophore lifetime. In comparison with one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. Combining with the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated the brighter and more contrast fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

UR - http://www.scopus.com/inward/record.url?scp=65949122727&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65949122727&partnerID=8YFLogxK

U2 - 10.1117/12.809134

DO - 10.1117/12.809134

M3 - Conference article

AN - SCOPUS:65949122727

VL - 7183

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

SN - 1605-7422

M1 - 71831L

ER -