Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

Ruei Yu He, Yuan Deng Su, Keng Chi Cho, Chun Yun Lin, Nan Shan Chang, Chih Han Chang, Shean Jen Chen

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)


A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide fluorescent images of living cell membranes. The proposed microscope with the help of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having a shorter fluorophore lifetime. In comparison with a one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. By combining the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated it's capability for brighter and more contrasted fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

Original languageEnglish
Pages (from-to)5987-5997
Number of pages11
JournalOptics Express
Issue number8
Publication statusPublished - 2009 Apr 13

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics


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