The effect of isoproterenol (Iso) on synaptic transmission mediated by the N-methyl-d-aspartate (NMDA) receptors (EPSPNMDA) was investigated in slices of rat amygdala using intracellular recording techniques. EPSPNMDA was isolated pharmacologically by application of a solution containing the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM) and GABAA receptor blocker bicuculline (20 μM). Superfusion of Iso (15 μM) produced a long-lasting enhancement of EPSPNMDA. Pretreatment the slices with propranolol (10 μM) completely prevented the effect of Iso confirming the mediation by β-adrenergic receptors. These results provide the direct evidence for adrenergic modulation of excitatory amino acid neurotransmission in the vertebrate central nervous system.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Clinical Neurology
- Developmental Biology