TY - JOUR
T1 - SWATH-MS in proteomics
T2 - Current status
AU - Wang, Jing
AU - Sheng, Quanhu
AU - Shyr, Yu
N1 - Publisher Copyright:
Copyright © 2015 Inderscience Enterprises Ltd.
PY - 2015
Y1 - 2015
N2 - Sequential window acquisition of all theoretical fragment ion spectra coupled to tandem mass spectrometry (SWATH-MS) is a new strategy for identification and quantification of proteins and proteomes proposed in 2012. SWATH-MS operates in unbiased data-independent acquisition (DIA) mode and combines the advantages of two widely used MS-based techniques: shotgun (high throughput) and SRM proteomics (high sensitivity and reproducibility). In this review, we provide an introduction of the SWATH-MS method, its application, and a perspective on the future of this powerful technology.
AB - Sequential window acquisition of all theoretical fragment ion spectra coupled to tandem mass spectrometry (SWATH-MS) is a new strategy for identification and quantification of proteins and proteomes proposed in 2012. SWATH-MS operates in unbiased data-independent acquisition (DIA) mode and combines the advantages of two widely used MS-based techniques: shotgun (high throughput) and SRM proteomics (high sensitivity and reproducibility). In this review, we provide an introduction of the SWATH-MS method, its application, and a perspective on the future of this powerful technology.
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U2 - 10.1504/IJCBDD.2015.072074
DO - 10.1504/IJCBDD.2015.072074
M3 - Article
AN - SCOPUS:84943395526
SN - 1756-0756
VL - 8
SP - 192
EP - 202
JO - International Journal of Computational Biology and Drug Design
JF - International Journal of Computational Biology and Drug Design
IS - 3
ER -