Abstract
We used a novel experimental setup to conduct the first synchrotron-based 61Ni Mössbauer spectroscopy measurements in the energy domain on Ni coordination complexes and metalloproteins. A representative set of samples was chosen to demonstrate the potential of this approach. 61NiCr2O4 was examined as a case with strong Zeeman splittings. Simulations of the spectra yielded an internal magnetic field of 44.6 T, consistent with previous work by the traditional 61Ni Mössbauer approach with a radioactive source. A linear Ni amido complex, 61Ni{N(SiMe3)Dipp}2, where Dipp = C6H3-2,6-iPr2, was chosen as a sample with an "extreme" geometry and large quadrupole splitting. Finally, to demonstrate the feasibility of metalloprotein studies using synchrotron-based 61Ni Mössbauer spectroscopy, we examined the spectra of 61Ni-substituted rubredoxin in reduced and oxidized forms, along with [Et4N]2[61Ni(SPh)4] as a model compound. For each of the above samples, a reasonable spectrum could be obtained in ∼1 d. Given that there is still room for considerable improvement in experimental sensitivity, synchrotron-based 61Ni Mössbauer spectroscopy appears to be a promising alternative to measurements with radioactive sources.
Original language | English |
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Pages (from-to) | 6866-6872 |
Number of pages | 7 |
Journal | Inorganic Chemistry |
Volume | 55 |
Issue number | 14 |
DOIs | |
Publication status | Published - 2016 Jul 18 |
All Science Journal Classification (ASJC) codes
- Physical and Theoretical Chemistry
- Inorganic Chemistry