Alkyne functionalized amphiphilic poly(amidoamine) (PAMAM) dendrimers were synthesized through surface modification and the metal ions (zirconium and titanium) were chelated on the surface of PAMAM dendrimer. Consequently, the synthesized nanomaterial and the bound phosphopeptide(s) were captured by the use of much popularized copper (I)-catalyzed click chemistry reaction to conjugate functionalized alkyne groups with poly(ethylene glycol) (PEG) containing azide groups. The characterization studies which were performed with FTIR and X-ray energy dispersive spectroscopy (EDS) revealed the functionalization of alkyne groups and the presence of chelated metal ions. The proposed method was successfully applied for the enrichment of phosphopeptides in simple peptide mixture and the results were analyzed by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF).
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Condensed Matter Physics
- Mechanics of Materials
- Mechanical Engineering