Abstract
Synthetic green fluorescent protein (GFP) chromophore analogues with a positive charge at the phenyl-like group have the highly electrophilic amidine carbon, smaller LUMO–HOMO energy gap, red-shifted electronic absorptions and fluorescent emissions, and accelerated E-Z thermoisomerization rates. They are water-labile and their hydrolysis results in ring-opening of the imidazolinone moiety with a half life around 25–37 h in D2O at 25 °C.
Original language | English |
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Pages (from-to) | 141-147 |
Number of pages | 7 |
Journal | Amino Acids |
Volume | 50 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2018 Jan 1 |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Clinical Biochemistry
- Organic Chemistry