TY - JOUR
T1 - Systemic steroid pretreatment improves cerebral protection after circulatory arrest
AU - Shum-Tim, Dominique
AU - Tchervenkov, Christo I.
AU - Jamal, Al Maleek
AU - Nimeh, Toni
AU - Luo, Chwan Yau
AU - Chedrawy, Edgar
AU - Laliberte, Eric
AU - Philip, Anie
AU - Rose, Colin P.
AU - Lavoie, Josee
N1 - Funding Information:
This research was performed with the support of a grant from the Heart and Stroke Foundation of Quebec and the Jonathan-Ballon Award granted to Dr Shum-Tim. We are also grateful for the support of the Andy Collins for Kids Foundation. The TGF-β 1 antibody was a gift donated by Dr O’Connor-McCourt from Biotechnology Research Institute, Montreal, Quebec, Canada.
PY - 2001
Y1 - 2001
N2 - Background. This study evaluates whether systemic steroid pretreatment enhances neuroprotection during deep hypothermic circulatory arrest (DHCA) compared with steroid in cardiopulmonary bypass (CPB) prime. Methods. Four-week-old piglets randomly placed into two groups (n = 5 per group) were given methylprednisolone (30 mg/kg) into the pump prime (group PP), or pretreated intravenously 4 hours before CPB (group PT). All animals underwent 100 minutes of DHCA (15°C), were weaned off CPB, and were sacrificed 6 hours later. Postoperative changes in body weight, bioimpedance, and colloid oncotic pressure (COP) were measured. Cerebral trypan blue content, immunohistochemical evaluation of transforming growth factor-β1 (TGF-βl) expression, and caspase-3 activity were performed. Results. Percentage weight gain (group PP 25.0% ± 10.4% versus group PT 12.5% ± 4.0%; p = 0.036), and percentage decrease in bioimpedance (PP 37.2% ± 14.5% versus PT 15.6% ± 7.9%; p = 0.019) were significantly lower, whereas postoperative COP was significantly higher in group PT versus group PP (PT 15,3 ± 1.8 mm Hg versus PP 11.6 ± 0.8 mm Hg; p = 0.003). Cerebral trypan blue (ng/g dry tissue) was significantly lower in group PT (PT 5.6 × 10-3 ± 1.1 × 10-3 versus PP 9.1 × 10-3 ± 5.7 × 10-4; p = 0.001). Increased TGF-β1 expression and decreased caspase-3 activity were shown in group PT. Conclusions. Systemic steroid pretreatment significantly reduced total body edema and cerebral vascular leak and was associated with better immunohistochemical indices of neuroprotection after DHCA.
AB - Background. This study evaluates whether systemic steroid pretreatment enhances neuroprotection during deep hypothermic circulatory arrest (DHCA) compared with steroid in cardiopulmonary bypass (CPB) prime. Methods. Four-week-old piglets randomly placed into two groups (n = 5 per group) were given methylprednisolone (30 mg/kg) into the pump prime (group PP), or pretreated intravenously 4 hours before CPB (group PT). All animals underwent 100 minutes of DHCA (15°C), were weaned off CPB, and were sacrificed 6 hours later. Postoperative changes in body weight, bioimpedance, and colloid oncotic pressure (COP) were measured. Cerebral trypan blue content, immunohistochemical evaluation of transforming growth factor-β1 (TGF-βl) expression, and caspase-3 activity were performed. Results. Percentage weight gain (group PP 25.0% ± 10.4% versus group PT 12.5% ± 4.0%; p = 0.036), and percentage decrease in bioimpedance (PP 37.2% ± 14.5% versus PT 15.6% ± 7.9%; p = 0.019) were significantly lower, whereas postoperative COP was significantly higher in group PT versus group PP (PT 15,3 ± 1.8 mm Hg versus PP 11.6 ± 0.8 mm Hg; p = 0.003). Cerebral trypan blue (ng/g dry tissue) was significantly lower in group PT (PT 5.6 × 10-3 ± 1.1 × 10-3 versus PP 9.1 × 10-3 ± 5.7 × 10-4; p = 0.001). Increased TGF-β1 expression and decreased caspase-3 activity were shown in group PT. Conclusions. Systemic steroid pretreatment significantly reduced total body edema and cerebral vascular leak and was associated with better immunohistochemical indices of neuroprotection after DHCA.
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U2 - 10.1016/S0003-4975(01)03129-0
DO - 10.1016/S0003-4975(01)03129-0
M3 - Article
C2 - 11722027
AN - SCOPUS:0035158148
SN - 0003-4975
VL - 72
SP - 1465
EP - 1472
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
IS - 5
ER -