T cell augments the antitumor activity of tumor-targeting Salmonella

Che Hsin Lee, Jeng Long Hsieh, Chao-Liang Wu, Pei Yu Hsu, Ai-Li Shiau

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Systemic administration of Salmonella to tumor-bearing mice leads to preferential accumulation within tumor sites and retardation of tumor growth. However, the detailed mechanism of Salmonella-induced antitumor immune response via host T cell remains uncertain. Herein, we used wild-type, CD4+ T-cell-deficient, and CD8+ T-cell-deficient mice to study the role of T cell in the antitumor immune responses induced by Salmonella enterica serovar Choleraesuis (Salmonella Choleraesuis). When systemically administered into mice bearing tumors, Salmonella Choleraesuis significantly inhibited tumor growth by 50%. In contrast, in T-cell-deficient mice, there was only 34-42% inhibition of tumor growth. We found that treatment with Salmonella Choleraesuis significantly upregulates interferon-γ in wild-type and CD8+ T-cell-deficient mice, but not in CD4+ T-cell-deficient mice. Furthermore, immunohistochemical staining of the tumors revealed more infiltration of macrophages and neutrophils in wild-type mice after Salmonella Choleraesuis treatment compared with those in T-cell-deficient mice. The antitumor therapeutic effect mediated by Salmonella Choleraesuis is associated with an inflammatory immune response at the tumor site and a tumor T helper 1-type immune response. In conclusion, these results suggest that tumor-targeted therapy using Salmonella Choleraesuis, which exerts tumoricidal effects and stimulates T cell activities, represents a potential strategy for the treatment of tumor.

Original languageEnglish
Pages (from-to)1381-1388
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume90
Issue number4
DOIs
Publication statusPublished - 2011 May 1

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Salmonella
T-Lymphocytes
Neoplasms
Growth
Salmonella enterica
Neutrophil Infiltration
Therapeutic Uses
Interferons
Up-Regulation
Macrophages
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Lee, Che Hsin ; Hsieh, Jeng Long ; Wu, Chao-Liang ; Hsu, Pei Yu ; Shiau, Ai-Li. / T cell augments the antitumor activity of tumor-targeting Salmonella. In: Applied Microbiology and Biotechnology. 2011 ; Vol. 90, No. 4. pp. 1381-1388.
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T cell augments the antitumor activity of tumor-targeting Salmonella. / Lee, Che Hsin; Hsieh, Jeng Long; Wu, Chao-Liang; Hsu, Pei Yu; Shiau, Ai-Li.

In: Applied Microbiology and Biotechnology, Vol. 90, No. 4, 01.05.2011, p. 1381-1388.

Research output: Contribution to journalArticle

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