TALE-based organellar genome editing and gene expression in plants

Jer Young Lin, Yu Chang Liu, Yan Hao Tseng, Ming Tsair Chan, Ching Chun Chang

Research output: Contribution to journalReview articlepeer-review

Abstract

Key message: TALE-based editors provide an alternative way to engineer the organellar genomes in plants. We update and discuss the most recent developments of TALE-based organellar genome editing in plants. Abstract: Gene editing tools have been widely used to modify the nuclear genomes of plants for various basic research and biotechnological applications. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 editing platform is the most commonly used technique because of its ease of use, fast speed, and low cost; however, it encounters difficulty when being delivered to plant organelles for gene editing. In contrast, protein-based editing technologies, such as transcription activator-like effector (TALE)-based tools, could be easily delivered, expressed, and targeted to organelles in plants via Agrobacteria-mediated nuclear transformation. Therefore, TALE-based editors provide an alternative way to engineer the organellar genomes in plants since the conventional chloroplast transformation method encounters technical challenges and is limited to certain species, and the direct transformation of mitochondria in higher plants is not yet possible. In this review, we update and discuss the most recent developments of TALE-based organellar genome editing in plants.

Original languageEnglish
Article number61
JournalPlant Cell Reports
Volume43
Issue number3
DOIs
Publication statusPublished - 2024 Mar

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

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