Temporal Differential Proteomes of Clostridium difficile in the Pig Ileal-Ligated Loop Model

Tavan Janvilisri, Joy Scaria, Ching-Hao Teng, Sean P. McDonough, Robin D. Gleed, Susan L. Fubini, Sheng Zhang, Bruce Akey, Yung Fu Chang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The impact of Clostridium difficile infection (CDI) on healthcare is becoming increasingly recognized as it represents a major cause of nosocomial diarrhea. A rising number of CDI cases and outbreaks have been reported worldwide. Here, we developed the pig ileal-ligated loop model for semi-quantitative analysis comparing temporal differential proteomes in C. difficile following in vivo incubation with in vitro growth using isobaric tags for relative and absolute quantification (iTRAQ). Proteins retrieved from the in vitro cultures and the loop contents after 4, 8, and 12 h in vivo incubation were subjected to in-solution digestion, iTRAQ labeling, two-dimensional liquid chromatography/tandem mass spectrometry and statistical analyses. From a total of 1152 distinct proteins identified in this study, 705 proteins were available for quantitative measures at all time points in both biological and technical replicates; 109 proteins were found to be differentially expressed. With analysis of clusters of orthologous group and protein-protein network interactions, we identified the proteins that might play roles in adaptive responses to the host environment, hence enhancing pathogenicity during CDI. This report represents the quantitative proteomic analysis of C. difficile that demonstrates time-dependent protein expression changes under conditions that mimic in vivo infection and identifies potential candidates for diagnostic or therapeutic measures.

Original languageEnglish
Article numbere45608
JournalPloS one
Volume7
Issue number9
DOIs
Publication statusPublished - 2012 Sep 18

Fingerprint

Clostridium difficile
Clostridium
Proteome
proteome
Swine
swine
Clostridium Infections
Proteins
proteins
infection
Protein Interaction Maps
Tandem Mass Spectrometry
Liquid chromatography
Liquid Chromatography
in vitro culture
Proteomics
proteomics
health services
liquid chromatography
Disease Outbreaks

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Janvilisri, T., Scaria, J., Teng, C-H., McDonough, S. P., Gleed, R. D., Fubini, S. L., ... Chang, Y. F. (2012). Temporal Differential Proteomes of Clostridium difficile in the Pig Ileal-Ligated Loop Model. PloS one, 7(9), [e45608]. https://doi.org/10.1371/journal.pone.0045608
Janvilisri, Tavan ; Scaria, Joy ; Teng, Ching-Hao ; McDonough, Sean P. ; Gleed, Robin D. ; Fubini, Susan L. ; Zhang, Sheng ; Akey, Bruce ; Chang, Yung Fu. / Temporal Differential Proteomes of Clostridium difficile in the Pig Ileal-Ligated Loop Model. In: PloS one. 2012 ; Vol. 7, No. 9.
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Janvilisri, T, Scaria, J, Teng, C-H, McDonough, SP, Gleed, RD, Fubini, SL, Zhang, S, Akey, B & Chang, YF 2012, 'Temporal Differential Proteomes of Clostridium difficile in the Pig Ileal-Ligated Loop Model', PloS one, vol. 7, no. 9, e45608. https://doi.org/10.1371/journal.pone.0045608

Temporal Differential Proteomes of Clostridium difficile in the Pig Ileal-Ligated Loop Model. / Janvilisri, Tavan; Scaria, Joy; Teng, Ching-Hao; McDonough, Sean P.; Gleed, Robin D.; Fubini, Susan L.; Zhang, Sheng; Akey, Bruce; Chang, Yung Fu.

In: PloS one, Vol. 7, No. 9, e45608, 18.09.2012.

Research output: Contribution to journalArticle

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