Temporal focusing-based widefield multiphoton microscopy with spatially modulated illumination for biotissue imaging

Chia Yuan Chang, Cheng Han Lin, Chun Yu Lin, Yong Da Sie, Yvonne Yuling Hu, Sheng Feng Tsai, Shean Jen Chen

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A developed temporal focusing-based multiphoton excitation microscope (TFMPEM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPEM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPEM can be condensed from 3.0 μm to 1.5 μm for a 50 % improvement. By using the TFMPEM with HiLo technique as two structured illuminations at the same spatial frequency but different orientation, reconstructed biotissue images according to the condensed AEC structured illumination are shown obviously superior in contrast and better scattering suppression. Picture: TPEF images of the eosin-stained mouse cerebellar cortex by conventional TFMPEM (left), and the TFMPEM with HiLo technique as 1.09 μm−1 spatially modulated illumination at 90° (center) and 0° (right) orientations. (Figure presented.).

Original languageEnglish
Article numbere201600287
JournalJournal of Biophotonics
Volume11
Issue number1
DOIs
Publication statusPublished - 2018 Jan

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Temporal focusing-based widefield multiphoton microscopy with spatially modulated illumination for biotissue imaging'. Together they form a unique fingerprint.

Cite this