TY - JOUR
T1 - Tepidimonas fonticaldi sp. nov., a slightly thermophilic betaproteobacterium isolated from a hot spring
AU - Chen, Wen Ming
AU - Huang, Hsing Wei
AU - Chang, Jo Shu
AU - Han, Yin Lung
AU - Guo, Tai Rong
AU - Sheu, Shih Yi
PY - 2013/5/1
Y1 - 2013/5/1
N2 - A slightly thermophilic bacterial strain, designated AT-A2T, was isolated from a hot spring water sample taken from the Antun hot spring in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain AT-A2Twere aerobic, Gram-negative, motile by a single polar flagellum and formed non-pigmented colonies. Growth occurred at 35-60 °C (optimum, 55 °C), with 0-1.0 % NaCl (optimum, 0.2 %) and at pH 7.0-9.0 (optimum, pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain AT-A2T belonged to the genus Tepidimonas and its closest neighbour wasTepidimonas thermarum AA-1T with a sequence similarity of 97.5 %. The predominant cellular fatty acids were C16: 0 (40.2 %), summed feature 3 (C16: 1ω7c and/or C16: 1ω6c; 20.1 %) and C17: 0 cyclo (11.5 %). The major respiratory quinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminolipid and several uncharacterized phospholipids. The DNA G+C content of strain AT-A2T was 70.1 mol%. The mean level of DNA-DNA relatedness between strain AT-A2T and Tepidimonas thermarum AA-1Twas 23.9 %. On the basis of the phylogenetic and phenotypic data, strain AT-A2T should be classified as representing a novel species, for which the nameTepidimonas fonticaldi sp. nov. is proposed. The type strain is AT-A2T (= LMG 26746T = KCTC 23862T = BCRC 80391T).
AB - A slightly thermophilic bacterial strain, designated AT-A2T, was isolated from a hot spring water sample taken from the Antun hot spring in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain AT-A2Twere aerobic, Gram-negative, motile by a single polar flagellum and formed non-pigmented colonies. Growth occurred at 35-60 °C (optimum, 55 °C), with 0-1.0 % NaCl (optimum, 0.2 %) and at pH 7.0-9.0 (optimum, pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain AT-A2T belonged to the genus Tepidimonas and its closest neighbour wasTepidimonas thermarum AA-1T with a sequence similarity of 97.5 %. The predominant cellular fatty acids were C16: 0 (40.2 %), summed feature 3 (C16: 1ω7c and/or C16: 1ω6c; 20.1 %) and C17: 0 cyclo (11.5 %). The major respiratory quinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminolipid and several uncharacterized phospholipids. The DNA G+C content of strain AT-A2T was 70.1 mol%. The mean level of DNA-DNA relatedness between strain AT-A2T and Tepidimonas thermarum AA-1Twas 23.9 %. On the basis of the phylogenetic and phenotypic data, strain AT-A2T should be classified as representing a novel species, for which the nameTepidimonas fonticaldi sp. nov. is proposed. The type strain is AT-A2T (= LMG 26746T = KCTC 23862T = BCRC 80391T).
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U2 - 10.1099/ijs.0.043729-0
DO - 10.1099/ijs.0.043729-0
M3 - Article
C2 - 22984136
AN - SCOPUS:84877863905
SN - 1466-5026
VL - 63
SP - 1810
EP - 1816
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - PART 5
ER -