Testosterone synthesized in cultured human SZ95 sebocytes derives mainly from dehydroepiandrosterone

Wen Chieh Chen, Shaw Jenq Tsai, Hamm Ming Sheu, Jih Chien Tsai, Christos C. Zouboulis

Research output: Contribution to journalLetterpeer-review

34 Citations (Scopus)

Abstract

Human sebaceous gland possesses all the steroidogenic enzymes required for androgen synthesis. It remains unclear whether the testosterone produced in situ mainly derives from circulating dehydroepiandrosterone (DHEA) or from de novo synthesis utilizing serum cholesterol. Using testosterone radioimmunoassay, we found that testosterone was barely detectable in the supernatant of cultured human SZ95 sebocytes when cholesterol was added alone, indicating a low basal expression of steroidogenic acute regulatory protein (StAR) in SZ95 cells. Human chorionic gonadotropin and fibroblast growth factor-9 were as potent as forskolin in activating StAR to enhance testosterone production, while interleukin-1β, dexamethasone, insulin and insulin-like growth factor-1 showed no stimulatory effect. A two-fold increase of testosterone production was observed in supplementation of DHEA as compared to pregnenolone, progesterone or 17α-hydroxyprogesterone. Based on our findings, testosterone synthesized in cultured sebocytes derived mainly from DHEA and inhibition of 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase may be a new target of androgen suppression for acne treatment.

Original languageEnglish
Pages (from-to)470-472
Number of pages3
JournalExperimental Dermatology
Volume19
Issue number5
DOIs
Publication statusPublished - 2010 May

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

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