We report the cloning and characterization of a chromosomal gene from Borrelia burgdorferi expressing an 83-kilodalton protein antigen in Escherichia coli. The antigen reacted strongly with antisera from two human Lyme disease patients. The chromosomal gene was expressed in a 6.5-kilobase-pair ClaI fragment cloned in a variable-reading-frame plasmid vector.
All Science Journal Classification (ASJC) codes
- Microbiology (medical)