The behavior of endothelial cells on polyurethane nanocomposites and the associated signaling pathways

Huey Shan Hung, Chia-Ching Wu, Shu Chien, Shan hui Hsu

Research output: Contribution to journalArticle

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Abstract

A series of nanocomposites from polyurethane (PU) incorporated with various low concentrations (17.4-174 ppm) of gold nanoparticles (approximately 5 nm) (denoted "PU-Au") were used as a model system to study the mechanisms that influenced endothelial cell (EC) migration on biomaterial surfaces. The migration rate of ECs on the PU-Au nanocomposites was determined by a real-time image system. It was found that ECs had the highest migration rate on the nanocomposite containing 43.5 ppm of gold ("PU-Au 43.5 ppm"). The high EC migration rate was associated with increased levels of endothelial nitric oxide synthase (eNOS) and phosphorylated-Akt (p-Akt) expressed by ECs cultured on PU-Au. The inductions of both eNOS and p-Akt on PU-Au were abolished by the addition of LY294002 (PI3K inhibitor), suggesting that these cellular events may be regulated through the PI3K signaling pathway. Using a biotinylated VEGF-165 that recognizes VEGF receptors and by FACS analysis, slightly higher expression of VEGF receptors for ECs on PU-Au was also demonstrated. Phalloidin staining showed that actin appeared as a circumferential band surrounding each cell on tissue culture polystyrene, whereas on PU-Au, especially on PU-Au 43.5 ppm, the cells had their margin spread out and extend processes with stress fibers in the protruding lamellipodia. Moreover, the higher EC migration rate on PU-Au 43.5 ppm was suppressed by LY294002. The higher protein expression of focal adhesion kinase (FAK) on PU-Au 43.5 ppm was observed in FAK-GFP transfected ECs. It was concluded that PU-Au nanocomposites activated FAK and the PI3K/Akt signaling pathway in ECs, leading to proliferation and migration of ECs on these surfaces.

Original languageEnglish
Pages (from-to)1502-1511
Number of pages10
JournalBiomaterials
Volume30
Issue number8
DOIs
Publication statusPublished - 2009 Mar 1

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Nanocomposites
Polyurethanes
Endothelial cells
Endothelial Cells
Focal Adhesion Protein-Tyrosine Kinases
Phosphatidylinositol 3-Kinases
Cell Movement
Vascular Endothelial Growth Factor Receptor
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Adhesion
Nitric Oxide Synthase Type III
Nitric oxide
Gold
Phalloidine
Tissue culture
Stress Fibers
Pseudopodia
Polystyrenes
Computer Systems
Biocompatible Materials

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Hung, Huey Shan ; Wu, Chia-Ching ; Chien, Shu ; Hsu, Shan hui. / The behavior of endothelial cells on polyurethane nanocomposites and the associated signaling pathways. In: Biomaterials. 2009 ; Vol. 30, No. 8. pp. 1502-1511.
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The behavior of endothelial cells on polyurethane nanocomposites and the associated signaling pathways. / Hung, Huey Shan; Wu, Chia-Ching; Chien, Shu; Hsu, Shan hui.

In: Biomaterials, Vol. 30, No. 8, 01.03.2009, p. 1502-1511.

Research output: Contribution to journalArticle

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