The binding of plasminogen fragments to cultured human umbilical vein endothelial cells

Hua Lin Wu, Ing Shiang Wu, Ro Yun Fang, Jen Shau Hau, Dung Ho Wu, Bi Ing Cheng, Tsun Mei Lin, Guey Yueh Shi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Glu-plasminogen, kringle 1-5, kringle 1-3; and miniplasminogen exhibited strong binding to human umbilical vein endothelial cells (HUVEC). On the other hand, no significant binding was obtained with microplasminogen and kringle 4. Kringle 1-5 and miniplasminogen, which both contained kringle 5, specifically inhibited the binding of plasminogen to HUVEC while kringle 1-3 did not. The results implied plasminogen molecule contained at least two binding sites, with which it interacted HUVEC. The stronger binding site was located in kringle 5 and the weaker one was in kringle 1-3. Kringle 4 and the active site domain exhibited no significant binding to HUVEC. The interaction of plasminogen with HUVEC is mainly through binding site on kringle 5.

Original languageEnglish
Pages (from-to)703-711
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume188
Issue number2
DOIs
Publication statusPublished - 1992 Oct 30

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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