The biosynthesis of N-arachidonoyl dopamine (NADA), a putative endocannabinoid and endovanilloid, via conjugation of arachidonic acid with dopamine

Sherry Shu Jung Hu, Heather B. Bradshaw, Valery M. Benton, Jay Shih Chieh Chen, Susan M. Huang, Alberto Minassi, Tiziana Bisogno, Kim Masuda, Bo Tan, Robert Roskoski, Benjamin F. Cravatt, Vincenzo Di Marzo, J. Michael Walker

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

N-arachidonoyl dopamine (NADA) is an endogenous ligand that activates the cannabinoid type 1 receptor and the transient receptor potential vanilloid type 1 channel. Two potential biosynthetic pathways for NADA have been proposed, though no conclusive evidence exists for either. The first is the direct conjugation of arachidonic acid with dopamine and the other is via metabolism of a putative N-arachidonoyl tyrosine (NA-tyrosine). In the present study we investigated these biosynthetic mechanisms and report that NADA synthesis requires TH in dopaminergic terminals; however, NA-tyrosine, which we identify here as an endogenous lipid, is not an intermediate. We show that NADA biosynthesis primarily occurs through an enzyme-mediated conjugation of arachidonic acid with dopamine. While this conjugation likely involves a complex of enzymes, our data suggest a direct involvement of fatty acid amide hydrolase in NADA biosynthesis either as a rate-limiting enzyme that liberates arachidonic acid from AEA, or as a conjugation enzyme, or both.

Original languageEnglish
Pages (from-to)291-301
Number of pages11
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume81
Issue number4
DOIs
Publication statusPublished - 2009 Oct

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology

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