Abstract
The biocompatibility of material plays an important role in the bone-implant interface for the prosthetic implant fixation. The biocompatibility of implants is associated with the chemical composition, surface topography, surface energy and surface roughness of biomaterials. The effects of two factors, surface roughness and serum contents, on osteoblast behavior at the surface of Ti-6AI-4V and plasma sprayed HA coating were investigated in the experiment. The osteoblasts derived from neonatal rat calvarial were cultured in Dulbecco's modified Eagle medium (DMEM) with fetal bovine serum (FBS) on the surface of polished Ti-6AI-4V (Ti-p), grit-blasted Ti-6AI-4V (Ti-b), polished HA coating (HAC-p), and as-sprayed HA coating (HAC). Under culture medium containing 4% FBS, the level of cell attachment to the polished surface is significantly higher than the rough surface of the same experimental materials during all culture periods. Increasing the contents of FBS up to 10%, the difference of osteoblast attachment is not found between Ti-p and Ti-b. Under 4% serum condition, the cell morphology attached to smooth surfaces (Ti-p and HAC-p) is spread faster and are more flattened than the one to rough surface of the same experimental materials by SEM. After 24h culture, the corroded cracks are easily observed at the surface of polished HA coatings, and the cell morphology on HAC-p coatings are elongated and less flattened compared with Ti-p. The result is consistent with statistical difference of cell attachment between Ti-p and HAC-p under 4% serum condition.
| Original language | English |
|---|---|
| Pages (from-to) | 341-350 |
| Number of pages | 10 |
| Journal | Journal of Materials Science: Materials in Medicine |
| Volume | 13 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2002 Apr 16 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Bioengineering
- Biomaterials
- Biomedical Engineering
Cite this
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The cell attachment and morphology of neonatal rat calvarial osteoblasts on the surface of Ti-6AI-4V and plasma-sprayed HA coating : Effect of surface roughness and serum contents. / Lee, Tzer-Min; Tsai, R. S.; Chang, E.; Yang, Chyun-Yu; Yang, M. R.
In: Journal of Materials Science: Materials in Medicine, Vol. 13, No. 4, 16.04.2002, p. 341-350.Research output: Contribution to journal › Article
TY - JOUR
T1 - The cell attachment and morphology of neonatal rat calvarial osteoblasts on the surface of Ti-6AI-4V and plasma-sprayed HA coating
T2 - Effect of surface roughness and serum contents
AU - Lee, Tzer-Min
AU - Tsai, R. S.
AU - Chang, E.
AU - Yang, Chyun-Yu
AU - Yang, M. R.
PY - 2002/4/16
Y1 - 2002/4/16
N2 - The biocompatibility of material plays an important role in the bone-implant interface for the prosthetic implant fixation. The biocompatibility of implants is associated with the chemical composition, surface topography, surface energy and surface roughness of biomaterials. The effects of two factors, surface roughness and serum contents, on osteoblast behavior at the surface of Ti-6AI-4V and plasma sprayed HA coating were investigated in the experiment. The osteoblasts derived from neonatal rat calvarial were cultured in Dulbecco's modified Eagle medium (DMEM) with fetal bovine serum (FBS) on the surface of polished Ti-6AI-4V (Ti-p), grit-blasted Ti-6AI-4V (Ti-b), polished HA coating (HAC-p), and as-sprayed HA coating (HAC). Under culture medium containing 4% FBS, the level of cell attachment to the polished surface is significantly higher than the rough surface of the same experimental materials during all culture periods. Increasing the contents of FBS up to 10%, the difference of osteoblast attachment is not found between Ti-p and Ti-b. Under 4% serum condition, the cell morphology attached to smooth surfaces (Ti-p and HAC-p) is spread faster and are more flattened than the one to rough surface of the same experimental materials by SEM. After 24h culture, the corroded cracks are easily observed at the surface of polished HA coatings, and the cell morphology on HAC-p coatings are elongated and less flattened compared with Ti-p. The result is consistent with statistical difference of cell attachment between Ti-p and HAC-p under 4% serum condition.
AB - The biocompatibility of material plays an important role in the bone-implant interface for the prosthetic implant fixation. The biocompatibility of implants is associated with the chemical composition, surface topography, surface energy and surface roughness of biomaterials. The effects of two factors, surface roughness and serum contents, on osteoblast behavior at the surface of Ti-6AI-4V and plasma sprayed HA coating were investigated in the experiment. The osteoblasts derived from neonatal rat calvarial were cultured in Dulbecco's modified Eagle medium (DMEM) with fetal bovine serum (FBS) on the surface of polished Ti-6AI-4V (Ti-p), grit-blasted Ti-6AI-4V (Ti-b), polished HA coating (HAC-p), and as-sprayed HA coating (HAC). Under culture medium containing 4% FBS, the level of cell attachment to the polished surface is significantly higher than the rough surface of the same experimental materials during all culture periods. Increasing the contents of FBS up to 10%, the difference of osteoblast attachment is not found between Ti-p and Ti-b. Under 4% serum condition, the cell morphology attached to smooth surfaces (Ti-p and HAC-p) is spread faster and are more flattened than the one to rough surface of the same experimental materials by SEM. After 24h culture, the corroded cracks are easily observed at the surface of polished HA coatings, and the cell morphology on HAC-p coatings are elongated and less flattened compared with Ti-p. The result is consistent with statistical difference of cell attachment between Ti-p and HAC-p under 4% serum condition.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036218091&partnerID=8YFLogxK
U2 - 10.1023/A:1014393815923
DO - 10.1023/A:1014393815923
M3 - Article
C2 - 15348608
AN - SCOPUS:0036218091
VL - 13
SP - 341
EP - 350
JO - Journal of Materials Science: Materials in Electronics
JF - Journal of Materials Science: Materials in Electronics
SN - 0957-4522
IS - 4
ER -