The development of behavioral and neuronal activities of animal models with kindling-induced and spontaneous temporal lobe seizures

Yu Lin Wang, Yin Lin Chen, Yu Shin Huang, Sheng-Fu Liang, Fu-Zen Shaw

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Epilepsy, in terms of the sudden and recurrent malfunction of brain caused by abnormal discharge, is called “seizure”. Recently, experiments on animal models have successfully demonstrated the better understanding of the excitability of neuronal networks in relation to the occurrence of seizures. In this work, the developments of behavioral and neuronal activities of kindling-induced and spontaneous temporal lobe epilepsy (TLE) in rats were investigated. Daily stimulation of the right-side amygdala was delivered to Wistar rats to induce TLE. Each kindling evokes the excited neuronal activities in the brain. The duration, spike density and amplitude of evoked discharges were increased during kindling procedure. With repeated 40 stimulations, the rats showed the progression from tonic seizures (stage-1,2) to generalized clonic- tonic convulsive seizures (stage-4,5). In a consequence, the daily stimulation causes the permanent brain function damage and three rats were successfully induced to have spontaneous TLE of seizure stage-2. The cortical neuronal activities exhibit high voltage rhythmic spike (HVRS) discharges oscillating in the rage of 9-11 Hz and 18-21 Hz (second harmonics). HVRS is characterized by a barrage of large spike discharge (80-400μV) with negative polarity. It was found that the inter-spike interval (ISI) between two negative spikes is positively correlated (r=0.9852) to the amplitude of former spike in a HVRS episode. A large amplitude spike was accompanied by a long ISI, while a small amplitude spike took place in group with a short ISI. The investigation on cortical epileptiform is practical to develop the on-line seizure detection algorithms. With integration of an electrical stimulator or drug delivery device, such a seizure warning system or a closed-loop seizure control device could enhance the patients’ life quality.

Original languageEnglish
Title of host publicationThe 15th International Conference on Biomedical Engineering, ICBME 2013
EditorsJames Goh
PublisherSpringer Verlag
Pages199-202
Number of pages4
ISBN (Electronic)9783319029122
DOIs
Publication statusPublished - 2014 Jan 1
Event15th International Conference on Biomedical Engineering, ICBME 2013 - Singapore, Singapore
Duration: 2013 Dec 42013 Dec 7

Publication series

NameIFMBE Proceedings
Volume43
ISSN (Print)1680-0737

Other

Other15th International Conference on Biomedical Engineering, ICBME 2013
CountrySingapore
CitySingapore
Period13-12-0413-12-07

Fingerprint

Rats
Animals
Brain
Electric potential
Alarm systems
Drug delivery
Experiments

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

Cite this

Wang, Y. L., Chen, Y. L., Huang, Y. S., Liang, S-F., & Shaw, F-Z. (2014). The development of behavioral and neuronal activities of animal models with kindling-induced and spontaneous temporal lobe seizures. In J. Goh (Ed.), The 15th International Conference on Biomedical Engineering, ICBME 2013 (pp. 199-202). (IFMBE Proceedings; Vol. 43). Springer Verlag. https://doi.org/10.1007/978-3-319-02913-9_51
Wang, Yu Lin ; Chen, Yin Lin ; Huang, Yu Shin ; Liang, Sheng-Fu ; Shaw, Fu-Zen. / The development of behavioral and neuronal activities of animal models with kindling-induced and spontaneous temporal lobe seizures. The 15th International Conference on Biomedical Engineering, ICBME 2013. editor / James Goh. Springer Verlag, 2014. pp. 199-202 (IFMBE Proceedings).
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abstract = "Epilepsy, in terms of the sudden and recurrent malfunction of brain caused by abnormal discharge, is called “seizure”. Recently, experiments on animal models have successfully demonstrated the better understanding of the excitability of neuronal networks in relation to the occurrence of seizures. In this work, the developments of behavioral and neuronal activities of kindling-induced and spontaneous temporal lobe epilepsy (TLE) in rats were investigated. Daily stimulation of the right-side amygdala was delivered to Wistar rats to induce TLE. Each kindling evokes the excited neuronal activities in the brain. The duration, spike density and amplitude of evoked discharges were increased during kindling procedure. With repeated 40 stimulations, the rats showed the progression from tonic seizures (stage-1,2) to generalized clonic- tonic convulsive seizures (stage-4,5). In a consequence, the daily stimulation causes the permanent brain function damage and three rats were successfully induced to have spontaneous TLE of seizure stage-2. The cortical neuronal activities exhibit high voltage rhythmic spike (HVRS) discharges oscillating in the rage of 9-11 Hz and 18-21 Hz (second harmonics). HVRS is characterized by a barrage of large spike discharge (80-400μV) with negative polarity. It was found that the inter-spike interval (ISI) between two negative spikes is positively correlated (r=0.9852) to the amplitude of former spike in a HVRS episode. A large amplitude spike was accompanied by a long ISI, while a small amplitude spike took place in group with a short ISI. The investigation on cortical epileptiform is practical to develop the on-line seizure detection algorithms. With integration of an electrical stimulator or drug delivery device, such a seizure warning system or a closed-loop seizure control device could enhance the patients’ life quality.",
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Wang, YL, Chen, YL, Huang, YS, Liang, S-F & Shaw, F-Z 2014, The development of behavioral and neuronal activities of animal models with kindling-induced and spontaneous temporal lobe seizures. in J Goh (ed.), The 15th International Conference on Biomedical Engineering, ICBME 2013. IFMBE Proceedings, vol. 43, Springer Verlag, pp. 199-202, 15th International Conference on Biomedical Engineering, ICBME 2013, Singapore, Singapore, 13-12-04. https://doi.org/10.1007/978-3-319-02913-9_51

The development of behavioral and neuronal activities of animal models with kindling-induced and spontaneous temporal lobe seizures. / Wang, Yu Lin; Chen, Yin Lin; Huang, Yu Shin; Liang, Sheng-Fu; Shaw, Fu-Zen.

The 15th International Conference on Biomedical Engineering, ICBME 2013. ed. / James Goh. Springer Verlag, 2014. p. 199-202 (IFMBE Proceedings; Vol. 43).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Wang YL, Chen YL, Huang YS, Liang S-F, Shaw F-Z. The development of behavioral and neuronal activities of animal models with kindling-induced and spontaneous temporal lobe seizures. In Goh J, editor, The 15th International Conference on Biomedical Engineering, ICBME 2013. Springer Verlag. 2014. p. 199-202. (IFMBE Proceedings). https://doi.org/10.1007/978-3-319-02913-9_51