@article{4c70458929094a798717efbb27138b50,
title = "The distinct role of STIM1 and STIM2 in the regulation of store-operated Ca2+ entry and cellular function",
abstract = "Stromal interaction molecules STIM1 and STIM2 are endoplasmic reticulum (ER) Ca2+ sensors that initiate store-operated Ca 2+ entry (SOCE). The roles of STIM1-mediated SOCE in cancer biology have been highlighted in different types of cancer, but that of STIM2 is unknown. By the model of cervical cancer, here we focus on the cooperative regulation of SOCE by STIM proteins and their distinct roles in cellular function. Immunofluorescent stainings of surgical specimens of cervical cancer show that STIM1 and STIM2 are abundant in tumor tissues, but STIM1 is the major ER Ca 2+ sensor identified in the invasive front of cancer tissues. STIM1 or STIM2 overexpression in cervical cancer SiHa cells induces an upregulated SOCE. Regarding cellular function, STIM1 and STIM2 are necessary for cell proliferation, whereas STIM1 is the dominant ER Ca 2+ sensor involved in cell migration. During SOCE, STIM1 is aggregated and translocated towards the Orai1-containing plasma membrane in association with the microtubule plus-end binding protein EB1. In contrast, STIM2 is constitutively aggregated without significant trafficking or association with microtubules. These results show the distinct role of STIM1 and STIM2 in SOCE and cellular function of cervical cancer cells.",
author = "Chen, \{Yih Fung\} and Chen, \{Li Hsien\} and Shen, \{Meng Ru\}",
note = "Funding Information: This study was partly supported by the Ministry of Science and Technology (MOST 104‐2320‐B‐006‐015‐MY3, MOST 106‐2319‐B‐ 006‐001, and MOST 107-2319-B-006-001 to M.‐R. S., and MOST 103‐2321‐B‐037‐002‐MY3 to Y.‐F. C.), National Health Research Institutes, Ministry of Health and Welfare (MOHW107-TDU-B-211-114018 and MOHW107-TDU-B-211-123003), National Cheng Kung University Hospital, Taiwan and Kaohsiung Medical University Research Foundation (KMU‐Q105008, KMU‐Q106009, and KMU‐ M107011 to Y.‐F. C.). We appreciate Misses Hsiao‐Tzu Chang and Lian‐Yun Chang for their technical assistance. We thank the technical services provided by the “Bioimaging Core Facility of the National Core Facility for Biopharmaceuticals, Ministry of Science and Technology, Taiwan.” Funding Information: This study was partly supported by the Ministry of Science and Technology (MOST 104-2320-B-006-015-MY3, MOST 106-2319-B-006-001, and MOST 107-2319-B-006-001 to M.-R. S., and MOST 103-2321-B-037-002-MY3 to Y.-F. C.), National Health Research Institutes, Ministry of Health and Welfare (MOHW107-TDU-B-211-114018 and MOHW107-TDU-B-211-123003), National Cheng Kung University Hospital, Taiwan and Kaohsiung Medical University Research Foundation (KMU-Q105008, KMU-Q106009, and KMU-M107011 to Y.-F. C.). We appreciate Misses Hsiao-Tzu Chang and Lian-Yun Chang for their technical assistance. We thank the technical services provided by the ?Bioimaging Core Facility of the National Core Facility for Biopharmaceuticals, Ministry of Science and Technology, Taiwan.? Publisher Copyright: {\textcopyright} 2018 Wiley Periodicals, Inc.",
year = "2019",
month = jun,
doi = "10.1002/jcp.27532",
language = "English",
volume = "234",
pages = "8727--8739",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "6",
}