The dual-specific binding of dengue virus and target cells for the antibody-dependent enhancement of dengue virus infection

Kao Jean Huang, Yu Ching Yang, Yee Shin Lin, Jyh Hsiung Huang, Hsiao Sheng Liu, Trai Ming Yeh, Shun Hua Chen, Ching Chuan Liu, Huan Yao Lei

Research output: Contribution to journalArticlepeer-review

143 Citations (Scopus)

Abstract

Using flow cytometric assay and monoclonal anti-dengue Ab, we observed that both anti-E and anti-prM Abs could enhance dengue virus infection in a concentration-dependent but serotype-independent manner. Increases were found in both the percentage of dengue-infected cells and the expression of dengue E and NS1 protein per cell. Dengue virion binding and infection were enhanced on FcR-bearing cells via the Fc-FcγRII pathway. Furthermore, anti-prM Ab also enhanced dengue virion binding and infection on cells lacking FcR, such as BHK-21 or A549 cells, by the mechanism of peptide (CPFLKQNEPEDIDCW)-specific binding. Anti-prM Ab cross-reacted with BHK-21 or A549 cells and recognized self-Ags such as heat shock protein 60. In summary, a novel mechanism of anti-prM Ab-mediated enhancement on dengue virus infection was found to be mediated by dual specific binding to dengue virion and to target cells, in addition to the traditional enhancement on FcR-bearing cells.

Original languageEnglish
Pages (from-to)2825-2832
Number of pages8
JournalJournal of Immunology
Volume176
Issue number5
DOIs
Publication statusPublished - 2006 Mar 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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