The dynamic responses of pro-inflammatory and anti-inflammatory cytokines of human mononuclear cells induced by uromodulin

Shu Jem Su, Trai Ming Yeh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

This study was undertaken to examine the dynamic response of human peripheral blood mononuclear cells (PBMC) in the secretion of pro-inflammatory and anti-inflammatory cytokines induced by uromodulin (URO). Levels of tumor necrosis factor-α (TNFα), TNF soluble receptor (sTNFRI and II), interleukin 1-β (IL-1β), and IL-1 receptor antagonist (IL-1Ra) in the supernatants of URO-stimulated PBMC were measured by ELISA. URO stimulated the secretion of all these cytokines in a dose dependent manner except sTNFRI. Peak levels of TNFα and IL-1β were reached at 6-12 h, while 5-10 fold higher in sTNFR II and IL-1Ra levels were observed at 24-48 h after URO stimulation. URO-induced secretion of TNFα, IL-1β, sTNFRII and IL-1Ra could be enhanced by human plasma. Specifically, serum proteins including C3, sCD14 and IgG not only bound to URO but also enhanced URO-induced TNFα secretion of PBMC. Collectively, our data suggest that URO might have dual immunomodulating effect through regulating the secretion of pro-inflammatory and anti-inflammatory cytokines, and that serum binding proteins might enhance this activity.

Original languageEnglish
Pages (from-to)2581-2590
Number of pages10
JournalLife Sciences
Volume65
Issue number24
DOIs
Publication statusPublished - 1999 Nov 5

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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