The effect of copper ion on arachidonic acid metabolism in the porcine corneal epithelium

Ming T. Lin, Yui Hsi Wang, Yuh Ling Chen, Wen Chang Chang

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5 Citations (Scopus)

Abstract

The effect of copper ion on arachidonic acid metabolism in the corneal epithelium was studied. Our results showed that when 3H-arachidonic acid (1 μCi/μg) was incubated with homogenate of corneal epithelium at 37°C for 45 min., three metabolites were formed. Metabolite A, the major metabolite, was identified as 12-HETE by HPLC and GC. An analysis with chiral phase HPLC revealed that metabolite A was a (R)enantiomer. The production of 12(R)-HETE was stimulated by low concentrations of CuCl2 (< 0.1 mM) and inhibited by higher concentrations of CuCl2. Study on the biological activity of 12(R)-HETE showed that it could stimulate migration of endothelial cells in a concentration as low as 10 ng/ml. These results suggest that one of the effects of copper ion on the corneal neovascularization may be to regulate the level of 12(R)-HETE.

Original languageEnglish
Pages (from-to)1122-1129
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume190
Issue number3
DOIs
Publication statusPublished - 1993 Feb 15

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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