The effect of copper ion on arachidonic acid metabolism in the porcine corneal epithelium

Ming T. Lin, Yui Hsi Wang, Yuh-Ling Chen, Wen Chang Chang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effect of copper ion on arachidonic acid metabolism in the corneal epithelium was studied. Our results showed that when 3H-arachidonic acid (1 μCi/μg) was incubated with homogenate of corneal epithelium at 37°C for 45 min., three metabolites were formed. Metabolite A, the major metabolite, was identified as 12-HETE by HPLC and GC. An analysis with chiral phase HPLC revealed that metabolite A was a (R)enantiomer. The production of 12(R)-HETE was stimulated by low concentrations of CuCl2 (< 0.1 mM) and inhibited by higher concentrations of CuCl2. Study on the biological activity of 12(R)-HETE showed that it could stimulate migration of endothelial cells in a concentration as low as 10 ng/ml. These results suggest that one of the effects of copper ion on the corneal neovascularization may be to regulate the level of 12(R)-HETE.

Original languageEnglish
Pages (from-to)1122-1129
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume190
Issue number3
DOIs
Publication statusPublished - 1993 Feb 15

Fingerprint

12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Corneal Epithelium
Metabolites
Arachidonic Acid
Metabolism
Copper
Swine
Ions
High Pressure Liquid Chromatography
Corneal Neovascularization
Enantiomers
Endothelial cells
Bioactivity
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

@article{9386c5ebf78e45a68735da20e00bdf4c,
title = "The effect of copper ion on arachidonic acid metabolism in the porcine corneal epithelium",
abstract = "The effect of copper ion on arachidonic acid metabolism in the corneal epithelium was studied. Our results showed that when 3H-arachidonic acid (1 μCi/μg) was incubated with homogenate of corneal epithelium at 37°C for 45 min., three metabolites were formed. Metabolite A, the major metabolite, was identified as 12-HETE by HPLC and GC. An analysis with chiral phase HPLC revealed that metabolite A was a (R)enantiomer. The production of 12(R)-HETE was stimulated by low concentrations of CuCl2 (< 0.1 mM) and inhibited by higher concentrations of CuCl2. Study on the biological activity of 12(R)-HETE showed that it could stimulate migration of endothelial cells in a concentration as low as 10 ng/ml. These results suggest that one of the effects of copper ion on the corneal neovascularization may be to regulate the level of 12(R)-HETE.",
author = "Lin, {Ming T.} and Wang, {Yui Hsi} and Yuh-Ling Chen and Chang, {Wen Chang}",
year = "1993",
month = "2",
day = "15",
doi = "10.1006/bbrc.1993.1166",
language = "English",
volume = "190",
pages = "1122--1129",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

The effect of copper ion on arachidonic acid metabolism in the porcine corneal epithelium. / Lin, Ming T.; Wang, Yui Hsi; Chen, Yuh-Ling; Chang, Wen Chang.

In: Biochemical and Biophysical Research Communications, Vol. 190, No. 3, 15.02.1993, p. 1122-1129.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of copper ion on arachidonic acid metabolism in the porcine corneal epithelium

AU - Lin, Ming T.

AU - Wang, Yui Hsi

AU - Chen, Yuh-Ling

AU - Chang, Wen Chang

PY - 1993/2/15

Y1 - 1993/2/15

N2 - The effect of copper ion on arachidonic acid metabolism in the corneal epithelium was studied. Our results showed that when 3H-arachidonic acid (1 μCi/μg) was incubated with homogenate of corneal epithelium at 37°C for 45 min., three metabolites were formed. Metabolite A, the major metabolite, was identified as 12-HETE by HPLC and GC. An analysis with chiral phase HPLC revealed that metabolite A was a (R)enantiomer. The production of 12(R)-HETE was stimulated by low concentrations of CuCl2 (< 0.1 mM) and inhibited by higher concentrations of CuCl2. Study on the biological activity of 12(R)-HETE showed that it could stimulate migration of endothelial cells in a concentration as low as 10 ng/ml. These results suggest that one of the effects of copper ion on the corneal neovascularization may be to regulate the level of 12(R)-HETE.

AB - The effect of copper ion on arachidonic acid metabolism in the corneal epithelium was studied. Our results showed that when 3H-arachidonic acid (1 μCi/μg) was incubated with homogenate of corneal epithelium at 37°C for 45 min., three metabolites were formed. Metabolite A, the major metabolite, was identified as 12-HETE by HPLC and GC. An analysis with chiral phase HPLC revealed that metabolite A was a (R)enantiomer. The production of 12(R)-HETE was stimulated by low concentrations of CuCl2 (< 0.1 mM) and inhibited by higher concentrations of CuCl2. Study on the biological activity of 12(R)-HETE showed that it could stimulate migration of endothelial cells in a concentration as low as 10 ng/ml. These results suggest that one of the effects of copper ion on the corneal neovascularization may be to regulate the level of 12(R)-HETE.

UR - http://www.scopus.com/inward/record.url?scp=0027210141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027210141&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1993.1166

DO - 10.1006/bbrc.1993.1166

M3 - Article

C2 - 8439312

AN - SCOPUS:0027210141

VL - 190

SP - 1122

EP - 1129

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -