TY - JOUR
T1 - The effect of prostacyclin agonists on the differentiation of phorbol ester treated human erythroleukemia cells
AU - Shen, Huang Wei
AU - Chen, Yu Lin
AU - Chern, Ching Yuh
AU - Kan, Wai Ming
N1 - Funding Information:
This work was supported in part by a grant from the Ministry of Education, Program for Promoting Academic Excellency of Universities, ROC (Grant number 91-B-FA09-1-4).
PY - 2007/5
Y1 - 2007/5
N2 - Phorbol-12-myristate-13-acetate (PMA) induces megakaryocytopoeisis in human erythroleukemia (HEL) cells which is characterized by the increase in cell size, increase in nuclear polyploidization and expression of megakaryocyte marker, CD41. However, upon treatment with 100 nM of selective prostacyclin (IP) agonist beraprost inhibits the induced differentiation. Moreover, selective non-prostanoid IP agonist, BMY 45778 prevents PMA induced megakaryocytopoeisis in HEL cells similarly, while prostaglandin E2 and specific EP3 agonist sulprostone have no effect. Thus, IP receptor is involved. Furthermore, adenylate cyclase activator forskolin and cAMP analog dibutyryl-cAMP also prevented PMA induced megakaryocytopoeisis in HEL cells. Thus, IP agonists inhibition of PMA induced megakaryocytopoeisis in HEL cells may involve a cAMP dependent pathway.
AB - Phorbol-12-myristate-13-acetate (PMA) induces megakaryocytopoeisis in human erythroleukemia (HEL) cells which is characterized by the increase in cell size, increase in nuclear polyploidization and expression of megakaryocyte marker, CD41. However, upon treatment with 100 nM of selective prostacyclin (IP) agonist beraprost inhibits the induced differentiation. Moreover, selective non-prostanoid IP agonist, BMY 45778 prevents PMA induced megakaryocytopoeisis in HEL cells similarly, while prostaglandin E2 and specific EP3 agonist sulprostone have no effect. Thus, IP receptor is involved. Furthermore, adenylate cyclase activator forskolin and cAMP analog dibutyryl-cAMP also prevented PMA induced megakaryocytopoeisis in HEL cells. Thus, IP agonists inhibition of PMA induced megakaryocytopoeisis in HEL cells may involve a cAMP dependent pathway.
UR - http://www.scopus.com/inward/record.url?scp=34247483420&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247483420&partnerID=8YFLogxK
U2 - 10.1016/j.prostaglandins.2007.01.012
DO - 10.1016/j.prostaglandins.2007.01.012
M3 - Article
C2 - 17481561
AN - SCOPUS:34247483420
SN - 1098-8823
VL - 83
SP - 231
EP - 236
JO - Journal of Lipid Mediators
JF - Journal of Lipid Mediators
IS - 3 SPEC. ISS.
ER -