The effect of toluene-substrate concentration on biological clogging and sloughing in porous media

W. C. Cheng, Tom Ming-Ching Kuo

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

One-dimensional flow experiments on biological clogging were carried out by biostimulating columns packed with glass beads, sterilized, and inoculated with toluene-utilizing bacteria. Biostimulation consisted of continuously injecting toluene at four concentrations (3.0 ± 0.9, 6.1 ± 0.8, 8.7 ± 1.6, and 11.3 ± 0.8 mg/L). The results of column flow experiments indicated that a threshold concentration of toluene exists below which the total biomass in the column can be kept at a steady-state level. The column cores were extruded and segmented to determine the biomass distribution throughout the column at clogging. Clogging resulted in a significant buildup of filamentous bacteria close to the inlet end. Based on the nucleotide sequence of 16S rRNA genes, the dominant filamentous bacteria were identified as Nocardia farcinica. A 235-d column experiment demonstrated that the clogging near injection points can be controlled by keeping the influent concentration of toluene below the threshold.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalGround Water Monitoring and Remediation
Volume30
Issue number4
DOIs
Publication statusPublished - 2010 Sep 1

All Science Journal Classification (ASJC) codes

  • Civil and Structural Engineering
  • Water Science and Technology

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