The effect of velocity and extensional strain rate on enhancing DNA hybridization

Yung Chiang Chung, Yu Cheng Lin, Yuh Lih Hsu, Wu Ning Tommy Chang, Ming Zheng Shiu

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


A study of the effect of fluidic velocities and extensional strain rates on DNA hybridization microchips was conducted. The hybridization efficiency could be improved by introducing velocity and extensional strain rate. Compared with conventional hybridization methods, this microchip was able to increase the hybridization signal nine-fold within 30 min. Three different devices were designed, fabricated and tested using 1.4 kb single stranded DNA as the target. Excellent correlation between simulation analysis and experimental data was obtained. Experimental results showed that the effect of extensional strain rate on the hybridization was larger than that of velocity. Based on this information, a new design of hybridization chip with microfluidic concepts of velocity and extensional strain rate may provide additional efficiency in DNA detection. This hybridization microchip can provide potential applications in genomic study in the future.

Original languageEnglish
Pages (from-to)1376-1383
Number of pages8
JournalJournal of Micromechanics and Microengineering
Issue number10
Publication statusPublished - 2004 Oct 1

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering


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