The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes

Yu Chu Wang, Kung-Chao Chang, Po-Wen Lin, Jenq-Chang Lee, Chien Hsien Lai, Li Jyuan Lin, Yun Yen, Chang Shen Lin, Shiang Jie Yang, Peng-Chan Lin, Chung-Ta Lee, Liang-Yi Hung

Research output: Contribution to journalArticle

Abstract

Heterogeneous nuclear ribonucleoprotein (hnRNP) Q1, an RNA-binding protein, has been implicated in many post-transcriptional processes, including RNA metabolism and mRNA splicing and translation. However, the role of hnRNP Q1 in tumorigenesis remains unclear. We previously performed RNA immunoprecipitation (RIP)-seq analysis to identify hnRNP Q1-interacting mRNAs and found that hnRNP Q1 targets a group of genes that are involved in mitotic regulation, including Aurora-A. Here, we demonstrate that altering the hnRNP Q1 level influences the expression of the Aurora-A protein, but not its mRNA. Stimulation with epidermal growth factor (EGF) enhances both binding between hnRNP Q1 and Aurora-A mRNA as well as the efficacy of the hnRNP Q1-induced translation of Aurora-A mRNA. The EGF/hnRNP Q1-induced translation of Aurora-A mRNA is mediated by the mTOR and ERK pathways. In addition, we show that hnRNP Q1 up-regulates the translation of a group of spindle assembly checkpoint (SAC) genes. hnRNP Q1 overexpression is positively correlated with the levels of Aurora-A and the SAC genes in human colorectal cancer tissues. In summary, our data suggest that hnRNP Q1 plays an important role in regulating the expression of a group of cell cycle-related genes. Therefore, it may contribute to tumorigenesis by up-regulating the translation of these genes in colorectal cancer.

Original languageEnglish
Article number70
JournalExperimental and Molecular Medicine
Volume50
Issue number6
DOIs
Publication statusPublished - 2018 Jun 1

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Heterogeneous-Nuclear Ribonucleoproteins
cdc Genes
Epidermal Growth Factor
Carcinogenesis
Genes
Cells
Messenger RNA
M Phase Cell Cycle Checkpoints
Colorectal Neoplasms
RNA
RNA-Binding Proteins
MAP Kinase Signaling System
Protein Biosynthesis
Immunoprecipitation
Metabolism
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

@article{4aa6c50e8f9a4284ab73c884d7b22cfd,
title = "The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes",
abstract = "Heterogeneous nuclear ribonucleoprotein (hnRNP) Q1, an RNA-binding protein, has been implicated in many post-transcriptional processes, including RNA metabolism and mRNA splicing and translation. However, the role of hnRNP Q1 in tumorigenesis remains unclear. We previously performed RNA immunoprecipitation (RIP)-seq analysis to identify hnRNP Q1-interacting mRNAs and found that hnRNP Q1 targets a group of genes that are involved in mitotic regulation, including Aurora-A. Here, we demonstrate that altering the hnRNP Q1 level influences the expression of the Aurora-A protein, but not its mRNA. Stimulation with epidermal growth factor (EGF) enhances both binding between hnRNP Q1 and Aurora-A mRNA as well as the efficacy of the hnRNP Q1-induced translation of Aurora-A mRNA. The EGF/hnRNP Q1-induced translation of Aurora-A mRNA is mediated by the mTOR and ERK pathways. In addition, we show that hnRNP Q1 up-regulates the translation of a group of spindle assembly checkpoint (SAC) genes. hnRNP Q1 overexpression is positively correlated with the levels of Aurora-A and the SAC genes in human colorectal cancer tissues. In summary, our data suggest that hnRNP Q1 plays an important role in regulating the expression of a group of cell cycle-related genes. Therefore, it may contribute to tumorigenesis by up-regulating the translation of these genes in colorectal cancer.",
author = "Wang, {Yu Chu} and Kung-Chao Chang and Po-Wen Lin and Jenq-Chang Lee and Lai, {Chien Hsien} and Lin, {Li Jyuan} and Yun Yen and Lin, {Chang Shen} and Yang, {Shiang Jie} and Peng-Chan Lin and Chung-Ta Lee and Liang-Yi Hung",
year = "2018",
month = "6",
day = "1",
doi = "10.1038/s12276-018-0101-6",
language = "English",
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The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes. / Wang, Yu Chu; Chang, Kung-Chao; Lin, Po-Wen; Lee, Jenq-Chang; Lai, Chien Hsien; Lin, Li Jyuan; Yen, Yun; Lin, Chang Shen; Yang, Shiang Jie; Lin, Peng-Chan; Lee, Chung-Ta; Hung, Liang-Yi.

In: Experimental and Molecular Medicine, Vol. 50, No. 6, 70, 01.06.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes

AU - Wang, Yu Chu

AU - Chang, Kung-Chao

AU - Lin, Po-Wen

AU - Lee, Jenq-Chang

AU - Lai, Chien Hsien

AU - Lin, Li Jyuan

AU - Yen, Yun

AU - Lin, Chang Shen

AU - Yang, Shiang Jie

AU - Lin, Peng-Chan

AU - Lee, Chung-Ta

AU - Hung, Liang-Yi

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Heterogeneous nuclear ribonucleoprotein (hnRNP) Q1, an RNA-binding protein, has been implicated in many post-transcriptional processes, including RNA metabolism and mRNA splicing and translation. However, the role of hnRNP Q1 in tumorigenesis remains unclear. We previously performed RNA immunoprecipitation (RIP)-seq analysis to identify hnRNP Q1-interacting mRNAs and found that hnRNP Q1 targets a group of genes that are involved in mitotic regulation, including Aurora-A. Here, we demonstrate that altering the hnRNP Q1 level influences the expression of the Aurora-A protein, but not its mRNA. Stimulation with epidermal growth factor (EGF) enhances both binding between hnRNP Q1 and Aurora-A mRNA as well as the efficacy of the hnRNP Q1-induced translation of Aurora-A mRNA. The EGF/hnRNP Q1-induced translation of Aurora-A mRNA is mediated by the mTOR and ERK pathways. In addition, we show that hnRNP Q1 up-regulates the translation of a group of spindle assembly checkpoint (SAC) genes. hnRNP Q1 overexpression is positively correlated with the levels of Aurora-A and the SAC genes in human colorectal cancer tissues. In summary, our data suggest that hnRNP Q1 plays an important role in regulating the expression of a group of cell cycle-related genes. Therefore, it may contribute to tumorigenesis by up-regulating the translation of these genes in colorectal cancer.

AB - Heterogeneous nuclear ribonucleoprotein (hnRNP) Q1, an RNA-binding protein, has been implicated in many post-transcriptional processes, including RNA metabolism and mRNA splicing and translation. However, the role of hnRNP Q1 in tumorigenesis remains unclear. We previously performed RNA immunoprecipitation (RIP)-seq analysis to identify hnRNP Q1-interacting mRNAs and found that hnRNP Q1 targets a group of genes that are involved in mitotic regulation, including Aurora-A. Here, we demonstrate that altering the hnRNP Q1 level influences the expression of the Aurora-A protein, but not its mRNA. Stimulation with epidermal growth factor (EGF) enhances both binding between hnRNP Q1 and Aurora-A mRNA as well as the efficacy of the hnRNP Q1-induced translation of Aurora-A mRNA. The EGF/hnRNP Q1-induced translation of Aurora-A mRNA is mediated by the mTOR and ERK pathways. In addition, we show that hnRNP Q1 up-regulates the translation of a group of spindle assembly checkpoint (SAC) genes. hnRNP Q1 overexpression is positively correlated with the levels of Aurora-A and the SAC genes in human colorectal cancer tissues. In summary, our data suggest that hnRNP Q1 plays an important role in regulating the expression of a group of cell cycle-related genes. Therefore, it may contribute to tumorigenesis by up-regulating the translation of these genes in colorectal cancer.

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