The expression of human antimicrobial peptide LL-37 in the human nasal mucosa

Po Hsu Chen, Sheen-Yie Fang

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Background: LL-37, an antimicrobial peptide, has been discovered to be produced by a number of epithelial cells. It is identified as a key element in the innate host defense mechanism. Because little is known about the expression of LL-37 in human sinonasal tract, we conducted this study to investigate the expression of LL-37 in human nasal mucosa. Methods: We investigated the expression of LL-37 gene by the reverse transcription polymerase chain reaction (RT-PCR) and localization of LL-37 peptide by immunohistochemistry in the inferior turbinate mucosa of 7 normal subjects and the nasal polyps of 12 patients with chronic paranasal sinusitis. Results: The transcripts of the LL-37 gene were detected in all human nasal tissues analyzed by RT-PCR. There is a significant increase of LL-37 mRNA expression in nasal polyps as compared with the normal nasal mucosa. Using immunohistochemistry, LL-37 peptide was localized in surface epithelial cells and submucosal glands. Conclusions: Our findings suggest that LL-37 is expressed by nasal mucosa and is upregulated during inflammation.

Original languageEnglish
Pages (from-to)381-385
Number of pages5
JournalAmerican Journal of Rhinology
Volume18
Issue number6
Publication statusPublished - 2004 Nov 1

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Nasal Mucosa
Nasal Polyps
Reverse Transcription
Epithelial Cells
Immunohistochemistry
Turbinates
Polymerase Chain Reaction
Sinusitis
Nose
Genes
Mucous Membrane
Inflammation
Messenger RNA
antimicrobial peptide LL-37
CAP18 lipopolysaccharide-binding protein

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

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The expression of human antimicrobial peptide LL-37 in the human nasal mucosa. / Chen, Po Hsu; Fang, Sheen-Yie.

In: American Journal of Rhinology, Vol. 18, No. 6, 01.11.2004, p. 381-385.

Research output: Contribution to journalArticle

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AU - Chen, Po Hsu

AU - Fang, Sheen-Yie

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AB - Background: LL-37, an antimicrobial peptide, has been discovered to be produced by a number of epithelial cells. It is identified as a key element in the innate host defense mechanism. Because little is known about the expression of LL-37 in human sinonasal tract, we conducted this study to investigate the expression of LL-37 in human nasal mucosa. Methods: We investigated the expression of LL-37 gene by the reverse transcription polymerase chain reaction (RT-PCR) and localization of LL-37 peptide by immunohistochemistry in the inferior turbinate mucosa of 7 normal subjects and the nasal polyps of 12 patients with chronic paranasal sinusitis. Results: The transcripts of the LL-37 gene were detected in all human nasal tissues analyzed by RT-PCR. There is a significant increase of LL-37 mRNA expression in nasal polyps as compared with the normal nasal mucosa. Using immunohistochemistry, LL-37 peptide was localized in surface epithelial cells and submucosal glands. Conclusions: Our findings suggest that LL-37 is expressed by nasal mucosa and is upregulated during inflammation.

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