The F-actin and adherence-dependent mechanical differentiation of normal epithelial cells after TGF-β1-induced EMT (tEMT) using a microplate measurement system

T. H. Wu, Y. W. Chiou, Wen-Tai Chiu, Ming-Jer Tang, C. H. Chen, Ming-Long Yeh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The epithelial to mesenchymal transition (EMT) is known to involve several physiological and pathological phenomena. In this study, we utilized a microplate measurement system (MMS) approach based on the deflection of a flexible micro-cantilever to measure cell stiffness (in Pa) and adhesion force (in nN) of a single cell during EMT with nN resolution. Our results demonstrated that after transforming growth factor-β1 (TGF-β1) induced EMT (tEMT), NMuMG cells became stiffer due to thicker and more abundant F-actin and displayed stronger vinculin accumulation after long-term cell-substrate adhesion. The MMS could distinguish differences in compressive stiffness (219±10 and 287±14 Pa), tensile stiffness (114±14 and 132±12 Pa), and adhesion force (150±42 and 192±31 nN) between cells before and after tEMT. However, without proper development of the F-actin structure and adequate adherent time, the mechanical differences were diminished. After tEMT, the cells with increased stiffness and a cell-substrate adhesion force benefited by migrating more rapidly and had more invasiveness. Thus, this technology has the potential to benefit research focused on cancer diagnosis, drug development, and cell-substrate interactions.

Original languageEnglish
Pages (from-to)465-478
Number of pages14
JournalBiomedical Microdevices
Volume16
Issue number3
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Epithelial-Mesenchymal Transition
Transforming Growth Factors
Actins
Adhesion
Epithelial Cells
Stiffness
Substrates
Cell Adhesion
Physiological Phenomena
Vinculin
Cell Communication
Intercellular Signaling Peptides and Proteins
Technology
Research
Pharmaceutical Preparations
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Molecular Biology

Cite this

@article{89987034b4c4457d958c4ce3ef28ae53,
title = "The F-actin and adherence-dependent mechanical differentiation of normal epithelial cells after TGF-β1-induced EMT (tEMT) using a microplate measurement system",
abstract = "The epithelial to mesenchymal transition (EMT) is known to involve several physiological and pathological phenomena. In this study, we utilized a microplate measurement system (MMS) approach based on the deflection of a flexible micro-cantilever to measure cell stiffness (in Pa) and adhesion force (in nN) of a single cell during EMT with nN resolution. Our results demonstrated that after transforming growth factor-β1 (TGF-β1) induced EMT (tEMT), NMuMG cells became stiffer due to thicker and more abundant F-actin and displayed stronger vinculin accumulation after long-term cell-substrate adhesion. The MMS could distinguish differences in compressive stiffness (219±10 and 287±14 Pa), tensile stiffness (114±14 and 132±12 Pa), and adhesion force (150±42 and 192±31 nN) between cells before and after tEMT. However, without proper development of the F-actin structure and adequate adherent time, the mechanical differences were diminished. After tEMT, the cells with increased stiffness and a cell-substrate adhesion force benefited by migrating more rapidly and had more invasiveness. Thus, this technology has the potential to benefit research focused on cancer diagnosis, drug development, and cell-substrate interactions.",
author = "Wu, {T. H.} and Chiou, {Y. W.} and Wen-Tai Chiu and Ming-Jer Tang and Chen, {C. H.} and Ming-Long Yeh",
year = "2014",
month = "1",
day = "1",
doi = "10.1007/s10544-014-9849-1",
language = "English",
volume = "16",
pages = "465--478",
journal = "Biomedical Microdevices",
issn = "1387-2176",
publisher = "Kluwer Academic Publishers",
number = "3",

}

TY - JOUR

T1 - The F-actin and adherence-dependent mechanical differentiation of normal epithelial cells after TGF-β1-induced EMT (tEMT) using a microplate measurement system

AU - Wu, T. H.

AU - Chiou, Y. W.

AU - Chiu, Wen-Tai

AU - Tang, Ming-Jer

AU - Chen, C. H.

AU - Yeh, Ming-Long

PY - 2014/1/1

Y1 - 2014/1/1

N2 - The epithelial to mesenchymal transition (EMT) is known to involve several physiological and pathological phenomena. In this study, we utilized a microplate measurement system (MMS) approach based on the deflection of a flexible micro-cantilever to measure cell stiffness (in Pa) and adhesion force (in nN) of a single cell during EMT with nN resolution. Our results demonstrated that after transforming growth factor-β1 (TGF-β1) induced EMT (tEMT), NMuMG cells became stiffer due to thicker and more abundant F-actin and displayed stronger vinculin accumulation after long-term cell-substrate adhesion. The MMS could distinguish differences in compressive stiffness (219±10 and 287±14 Pa), tensile stiffness (114±14 and 132±12 Pa), and adhesion force (150±42 and 192±31 nN) between cells before and after tEMT. However, without proper development of the F-actin structure and adequate adherent time, the mechanical differences were diminished. After tEMT, the cells with increased stiffness and a cell-substrate adhesion force benefited by migrating more rapidly and had more invasiveness. Thus, this technology has the potential to benefit research focused on cancer diagnosis, drug development, and cell-substrate interactions.

AB - The epithelial to mesenchymal transition (EMT) is known to involve several physiological and pathological phenomena. In this study, we utilized a microplate measurement system (MMS) approach based on the deflection of a flexible micro-cantilever to measure cell stiffness (in Pa) and adhesion force (in nN) of a single cell during EMT with nN resolution. Our results demonstrated that after transforming growth factor-β1 (TGF-β1) induced EMT (tEMT), NMuMG cells became stiffer due to thicker and more abundant F-actin and displayed stronger vinculin accumulation after long-term cell-substrate adhesion. The MMS could distinguish differences in compressive stiffness (219±10 and 287±14 Pa), tensile stiffness (114±14 and 132±12 Pa), and adhesion force (150±42 and 192±31 nN) between cells before and after tEMT. However, without proper development of the F-actin structure and adequate adherent time, the mechanical differences were diminished. After tEMT, the cells with increased stiffness and a cell-substrate adhesion force benefited by migrating more rapidly and had more invasiveness. Thus, this technology has the potential to benefit research focused on cancer diagnosis, drug development, and cell-substrate interactions.

UR - http://www.scopus.com/inward/record.url?scp=84904158957&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904158957&partnerID=8YFLogxK

U2 - 10.1007/s10544-014-9849-1

DO - 10.1007/s10544-014-9849-1

M3 - Article

C2 - 24627216

AN - SCOPUS:84904158957

VL - 16

SP - 465

EP - 478

JO - Biomedical Microdevices

JF - Biomedical Microdevices

SN - 1387-2176

IS - 3

ER -