The F-actin and adherence-dependent mechanical differentiation of normal epithelial cells after TGF-β1-induced EMT (tEMT) using a microplate measurement system

T. H. Wu, Y. W. Chiou, W. T. Chiu, M. J. Tang, C. H. Chen, Ming Long Yeh

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The epithelial to mesenchymal transition (EMT) is known to involve several physiological and pathological phenomena. In this study, we utilized a microplate measurement system (MMS) approach based on the deflection of a flexible micro-cantilever to measure cell stiffness (in Pa) and adhesion force (in nN) of a single cell during EMT with nN resolution. Our results demonstrated that after transforming growth factor-β1 (TGF-β1) induced EMT (tEMT), NMuMG cells became stiffer due to thicker and more abundant F-actin and displayed stronger vinculin accumulation after long-term cell-substrate adhesion. The MMS could distinguish differences in compressive stiffness (219±10 and 287±14 Pa), tensile stiffness (114±14 and 132±12 Pa), and adhesion force (150±42 and 192±31 nN) between cells before and after tEMT. However, without proper development of the F-actin structure and adequate adherent time, the mechanical differences were diminished. After tEMT, the cells with increased stiffness and a cell-substrate adhesion force benefited by migrating more rapidly and had more invasiveness. Thus, this technology has the potential to benefit research focused on cancer diagnosis, drug development, and cell-substrate interactions.

Original languageEnglish
Pages (from-to)465-478
Number of pages14
JournalBiomedical Microdevices
Volume16
Issue number3
DOIs
Publication statusPublished - 2014 Jun

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Molecular Biology

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