TY - JOUR
T1 - The functional property of royal jelly 10-hydroxy-2-decenoic acid as a melanogenesis inhibitor
AU - Peng, Chi Chung
AU - Sun, Hui Tzu
AU - Lin, I. Ping
AU - Kuo, Ping Chung
AU - Li, Jen Chieh
N1 - Funding Information:
We thank Ms. Li-Yu Lee of the Fu-Chang Beekeeping for preparing royal jelly. This work was supported by a grant from the Ministry of Science and Technology, Taiwan, ROC (MOST102-2622-B-150-002-CC2 & MOST 103-2313-B-150 -001 -MY2 to C. C. Peng).
PY - 2017/8/9
Y1 - 2017/8/9
N2 - Background: It has been reported that royal jelly would reduce melanin synthesis and inhibit the expression of melanogensis related proteins and genes. In this study, we evaluate the anti-melanogenic and depigmenting activity of 10-hydroxy-2-decenoic acid (10-HDA) from royal jelly of Apis mellifera. Methods: In this study, we assesses the 10-HDA whitening activity in comparison with the changes in the intracellular tyrosinase activity, melanin content and melanin production related protein levles in B16F1 melanoma cells after treating with 10-HDA. Furthermore, the skin whitening effect was evaluated by applying a cream product containing with 0.5%, 1% and 2% of 10-HDA onto the skin of mice (C57BL/6J) for 3week to observe the effect of DL*-values. Results: The results showed that 10-HDA inhibited the MITF protein expression (IC50 0.86mM) in B16F1 melanoma cells. Western blot analysis revealed that 10-HDA inhibited the activity of tyrosinase and the expression of tyrosinase-related protein 1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF) in B16F1 melanoma cells. In addition, the 10-HDA was applied on the skin of mice show significantly increased the average skin-whitening index (L value). Conclusions: The validation data indicated the potential of 10-HDA for use in suppressing skin pigmentation. The 10-HDA is proposed as a candidate to inhibit melanogenesis, thus it could be developed as cosmetics skin care products.
AB - Background: It has been reported that royal jelly would reduce melanin synthesis and inhibit the expression of melanogensis related proteins and genes. In this study, we evaluate the anti-melanogenic and depigmenting activity of 10-hydroxy-2-decenoic acid (10-HDA) from royal jelly of Apis mellifera. Methods: In this study, we assesses the 10-HDA whitening activity in comparison with the changes in the intracellular tyrosinase activity, melanin content and melanin production related protein levles in B16F1 melanoma cells after treating with 10-HDA. Furthermore, the skin whitening effect was evaluated by applying a cream product containing with 0.5%, 1% and 2% of 10-HDA onto the skin of mice (C57BL/6J) for 3week to observe the effect of DL*-values. Results: The results showed that 10-HDA inhibited the MITF protein expression (IC50 0.86mM) in B16F1 melanoma cells. Western blot analysis revealed that 10-HDA inhibited the activity of tyrosinase and the expression of tyrosinase-related protein 1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF) in B16F1 melanoma cells. In addition, the 10-HDA was applied on the skin of mice show significantly increased the average skin-whitening index (L value). Conclusions: The validation data indicated the potential of 10-HDA for use in suppressing skin pigmentation. The 10-HDA is proposed as a candidate to inhibit melanogenesis, thus it could be developed as cosmetics skin care products.
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U2 - 10.1186/s12906-017-1888-8
DO - 10.1186/s12906-017-1888-8
M3 - Article
C2 - 28793915
AN - SCOPUS:85027122223
VL - 17
JO - BMC Complementary and Alternative Medicine
JF - BMC Complementary and Alternative Medicine
SN - 1472-6882
IS - 1
M1 - 392
ER -