Abstract
Background: It has been reported that royal jelly would reduce melanin synthesis and inhibit the expression of melanogensis related proteins and genes. In this study, we evaluate the anti-melanogenic and depigmenting activity of 10-hydroxy-2-decenoic acid (10-HDA) from royal jelly of Apis mellifera. Methods: In this study, we assesses the 10-HDA whitening activity in comparison with the changes in the intracellular tyrosinase activity, melanin content and melanin production related protein levles in B16F1 melanoma cells after treating with 10-HDA. Furthermore, the skin whitening effect was evaluated by applying a cream product containing with 0.5%, 1% and 2% of 10-HDA onto the skin of mice (C57BL/6J) for 3week to observe the effect of DL*-values. Results: The results showed that 10-HDA inhibited the MITF protein expression (IC50 0.86mM) in B16F1 melanoma cells. Western blot analysis revealed that 10-HDA inhibited the activity of tyrosinase and the expression of tyrosinase-related protein 1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF) in B16F1 melanoma cells. In addition, the 10-HDA was applied on the skin of mice show significantly increased the average skin-whitening index (L value). Conclusions: The validation data indicated the potential of 10-HDA for use in suppressing skin pigmentation. The 10-HDA is proposed as a candidate to inhibit melanogenesis, thus it could be developed as cosmetics skin care products.
| Original language | English |
|---|---|
| Article number | 392 |
| Journal | BMC Complementary and Alternative Medicine |
| Volume | 17 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2017 Aug 9 |
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All Science Journal Classification (ASJC) codes
- Complementary and alternative medicine
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The functional property of royal jelly 10-hydroxy-2-decenoic acid as a melanogenesis inhibitor. / Peng, Chi Chung; Sun, Hui Tzu; Lin, I. Ping; Kuo, Ping-Chung; Li, Jen Chieh.
In: BMC Complementary and Alternative Medicine, Vol. 17, No. 1, 392, 09.08.2017.Research output: Contribution to journal › Article
TY - JOUR
T1 - The functional property of royal jelly 10-hydroxy-2-decenoic acid as a melanogenesis inhibitor
AU - Peng, Chi Chung
AU - Sun, Hui Tzu
AU - Lin, I. Ping
AU - Kuo, Ping-Chung
AU - Li, Jen Chieh
PY - 2017/8/9
Y1 - 2017/8/9
N2 - Background: It has been reported that royal jelly would reduce melanin synthesis and inhibit the expression of melanogensis related proteins and genes. In this study, we evaluate the anti-melanogenic and depigmenting activity of 10-hydroxy-2-decenoic acid (10-HDA) from royal jelly of Apis mellifera. Methods: In this study, we assesses the 10-HDA whitening activity in comparison with the changes in the intracellular tyrosinase activity, melanin content and melanin production related protein levles in B16F1 melanoma cells after treating with 10-HDA. Furthermore, the skin whitening effect was evaluated by applying a cream product containing with 0.5%, 1% and 2% of 10-HDA onto the skin of mice (C57BL/6J) for 3week to observe the effect of DL*-values. Results: The results showed that 10-HDA inhibited the MITF protein expression (IC50 0.86mM) in B16F1 melanoma cells. Western blot analysis revealed that 10-HDA inhibited the activity of tyrosinase and the expression of tyrosinase-related protein 1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF) in B16F1 melanoma cells. In addition, the 10-HDA was applied on the skin of mice show significantly increased the average skin-whitening index (L value). Conclusions: The validation data indicated the potential of 10-HDA for use in suppressing skin pigmentation. The 10-HDA is proposed as a candidate to inhibit melanogenesis, thus it could be developed as cosmetics skin care products.
AB - Background: It has been reported that royal jelly would reduce melanin synthesis and inhibit the expression of melanogensis related proteins and genes. In this study, we evaluate the anti-melanogenic and depigmenting activity of 10-hydroxy-2-decenoic acid (10-HDA) from royal jelly of Apis mellifera. Methods: In this study, we assesses the 10-HDA whitening activity in comparison with the changes in the intracellular tyrosinase activity, melanin content and melanin production related protein levles in B16F1 melanoma cells after treating with 10-HDA. Furthermore, the skin whitening effect was evaluated by applying a cream product containing with 0.5%, 1% and 2% of 10-HDA onto the skin of mice (C57BL/6J) for 3week to observe the effect of DL*-values. Results: The results showed that 10-HDA inhibited the MITF protein expression (IC50 0.86mM) in B16F1 melanoma cells. Western blot analysis revealed that 10-HDA inhibited the activity of tyrosinase and the expression of tyrosinase-related protein 1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF) in B16F1 melanoma cells. In addition, the 10-HDA was applied on the skin of mice show significantly increased the average skin-whitening index (L value). Conclusions: The validation data indicated the potential of 10-HDA for use in suppressing skin pigmentation. The 10-HDA is proposed as a candidate to inhibit melanogenesis, thus it could be developed as cosmetics skin care products.
UR - http://www.scopus.com/inward/record.url?scp=85027122223&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027122223&partnerID=8YFLogxK
U2 - 10.1186/s12906-017-1888-8
DO - 10.1186/s12906-017-1888-8
M3 - Article
C2 - 28793915
AN - SCOPUS:85027122223
VL - 17
JO - BMC Complementary and Alternative Medicine
JF - BMC Complementary and Alternative Medicine
SN - 1472-6882
IS - 1
M1 - 392
ER -