The General Characteristics of a Birnavirus Isolated from Cultured Loach(Misgurnus anguillicaudatus) in Taiwan

H. Y. Chou, Chu-Fang Lo, M. C. Tung, C. H. Wang, H. Fukuda, T. Sano

Research output: Contribution to journalArticle

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Abstract

A birnavirus was isolated from cultured loach (Misgurnus anguillicaudatus) in Taiwan with TO-2 cell line. Evident cytopathic effects of the virus, including karyopycnosis, cytoplasmic inclusion bodies and cell destruction, were observed in TO-2 cells by light microscopy. The naked icosahedral virion layered by a single capsid with a diameter of 69±4 nm was observed by electron microscopy. Sometimes, complete virions were found to be enclosed by lysosomes to form cytolysome. The virus propagated in several fish cell lines and its replication cycle was completed in 24 hours at its optimum multiplication temperature of 25°C. This virus has a two segmented double-stranded RNA genome and 3 structural proteins, showing 1.33 g/cm3 buoyant density in CsCl. The chemico-physical properties of the virus together with the result of cross neutralization test indicated that it is a birnavirus and is closely related to Ab serotype of infectious pancreatic necrosis virus (IPNV).

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalFish Pathology
Volume28
Issue number1
DOIs
Publication statusPublished - 1993 Jan 1

Fingerprint

Birnaviridae
Misgurnus anguillicaudatus
Cobitidae
general characteristics
Taiwan
virus
viruses
virion
cell lines
Infectious pancreatic necrosis virus
cytoplasmic inclusions
cytopathogenicity
capsid
structural proteins
double-stranded RNA
inclusion bodies
lysosomes
neutralization tests
light microscopy
neutralization

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Animal Science and Zoology

Cite this

Chou, H. Y. ; Lo, Chu-Fang ; Tung, M. C. ; Wang, C. H. ; Fukuda, H. ; Sano, T. / The General Characteristics of a Birnavirus Isolated from Cultured Loach(Misgurnus anguillicaudatus) in Taiwan. In: Fish Pathology. 1993 ; Vol. 28, No. 1. pp. 1-7.
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The General Characteristics of a Birnavirus Isolated from Cultured Loach(Misgurnus anguillicaudatus) in Taiwan. / Chou, H. Y.; Lo, Chu-Fang; Tung, M. C.; Wang, C. H.; Fukuda, H.; Sano, T.

In: Fish Pathology, Vol. 28, No. 1, 01.01.1993, p. 1-7.

Research output: Contribution to journalArticle

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