The influence of surface morphology and rigidity of the substrata on cell motility

Chien Chang Chen, Patrick Chin Ho Hsieh, Guo Ming Wang, Wen Chi Chen, Ming Long Yeh

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The purpose of this study is to measure the cell motility under different stiffness and pattern of the substrata. Human melanoma cells were used for this study. Three surface patterns including flat, 6 μm-cone, and 6 μm-groove on polydimethylsiloxane (PDMS) were created by the micro-electro-mechanical system (MEMS). Glass dish was used as control substrate. After cells were seeded for 30 min, the cell images were taken every minute for 2 h. Each group had 4-5 dishes and 27-38 cells were calculated. The cell motility was 1.13 ± 1.30, 2.12 ± 1.21, 2.39 ± 1.11 and 3.08 ± 1.49 μm/min on glass, flat, cone, and groove PDMS, respectively. Cells in PDMS groups moved significantly faster than the control group (glass) due to smaller stiffness of the former substrates. More than 80% of cells on grooved PDMS moved along the grooves, indicating the grooved surface morphology could control the direction of cell movement. Our results display that substrate modulus and pattern can influence cell motility.

Original languageEnglish
Pages (from-to)1872-1875
Number of pages4
JournalMaterials Letters
Volume63
Issue number21
DOIs
Publication statusPublished - 2009 Aug 31

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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