The innate immune response and activation of coagulation in ±1,3-galactosyltransferase gene-knockout xenograft recipients

Mohamed Ezzelarab, Bertha Garcia, Agnes Azimzadeh, Hongtao Sun, Chih Che Lin, Hidetaka Hara, Sean Kelishadi, Tianshu Zhang, Yih Jyh Lin, Hao Chi Tai, Robert Wagner, Jnanesh Thacker, Noriko Murase, Kenneth McCurry, Rolf N. Barth, David Ayares, Richard N. Pierson, David K.C. Cooper

Research output: Contribution to journalArticlepeer-review

113 Citations (Scopus)

Abstract

BACKGROUND.: The role of the innate immune system in the development of thrombotic microangiopathy (TM) afterα1,3-galactosyltransferase gene-knockout (GTKO) pig organ transplantation in primates is uncertain. METHODS.: Twelve organs (nine hearts, three kidneys) from GTKO pigs were transplanted into baboons that received no immunosuppressive therapy, partial regimens, or a full regimen based on costimulation blockade. After graft failure, histologic and immunohistologic examinations were carried out. RESULTS.: Graft survival of less than 1 day was prolonged to 2 to 12 days with partial regimens (acute humoral xenograft rejection) and to 5 and 8 weeks with the full regimen (TM). Clinical or laboratory features of consumptive coagulopathy occurred in 7 of 12 baboons. Immunohistochemistry demonstrated IgM, IgG, and complement deposition in most cases. Histopathology demonstrated neutrophil and macrophage infiltrates, intravascular fibrin deposition, and platelet aggregation (TM). Grafts showed expression of primate tissue factor (TF), with increased mRNA levels, and TF was also expressed on baboon macrophages/monocytes infiltrating the graft. CONCLUSIONS.: Our data suggest that (1) irrespective of the presence or absence of the adaptive immune response, early or late xenograft rejection is associated with activation of the innate immune system; and (2) porcine endothelial cell activation and primate TF expression by recipient innate immune cells may both contribute to the development of TM.

Original languageEnglish
Pages (from-to)805-812
Number of pages8
JournalTransplantation
Volume87
Issue number6
DOIs
Publication statusPublished - 2009 Mar 27

All Science Journal Classification (ASJC) codes

  • Transplantation

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