Abstract

Poor oral hygiene may lead to overgrowth of pathogenic oral bacteria, which may induce chronic inflammation to promote the oncogenesis of oral squamous cell carcinoma (OSCC). This study investigated the association between oral bacterial profile and OSCC risk in a case-control study of 138 OSCC cases and 151 controls (88 cases and 90 controls for the discovery group and 50 cases and 61 controls for the validation group). Oral bacterial profiles were characterized by targeted sequencing of the 16S rRNA gene. Three species of periodontopathogenic bacteria, Prevotella tannerae, Fusobacterium nucleatum, and Prevotella intermedia, were associated with an increased OSCC risk. This association was modified by the genetic polymorphisms of TLR2 and TLR4. Use of alcohol, betel quids and cigarettes and poor oral hygiene were associated with a higher percentage of oral periodontopathogenic bacteria. The association between alcohol and periodontopathogenic bacteria was modified by the genetic polymorphism of ALDH2, with a stronger positive association observed among the ALDH2-deficient individuals. The percentage of periodontopathogenic bacteria was positively correlated with the level of salivary IL1β, an inflammatory cytokine. Overall, our results showed a positive association between periodontopathogenic bacteria and OSCC risk and this relationship may be influenced by lifestyle and genetic factors. Our results provided further biological support for the established association between poor oral hygiene and OSCC risk. This suggested that improving oral hygiene may reduce OSCC risk and should be part of a public health campaign to prevent the occurrence of OSCC.

Original languageEnglish
Pages (from-to)778-787
Number of pages10
JournalCarcinogenesis
Volume39
Issue number6
DOIs
Publication statusPublished - 2018 May 28

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Microbiota
Genetic Polymorphisms
Life Style
Squamous Cell Carcinoma
Oral Hygiene
Bacteria
Alcohols
Prevotella intermedia
Prevotella
Fusobacterium nucleatum
Control Groups
Health Promotion
rRNA Genes
Tobacco Products
Case-Control Studies
Carcinogenesis
Public Health
Cytokines
Inflammation

All Science Journal Classification (ASJC) codes

  • Cancer Research

Cite this

@article{b3de87dd27124c79b4cd3ba0a15dabc9,
title = "The interplay between oral microbiome, lifestyle factors and genetic polymorphisms in the risk of oral squamous cell carcinoma",
abstract = "Poor oral hygiene may lead to overgrowth of pathogenic oral bacteria, which may induce chronic inflammation to promote the oncogenesis of oral squamous cell carcinoma (OSCC). This study investigated the association between oral bacterial profile and OSCC risk in a case-control study of 138 OSCC cases and 151 controls (88 cases and 90 controls for the discovery group and 50 cases and 61 controls for the validation group). Oral bacterial profiles were characterized by targeted sequencing of the 16S rRNA gene. Three species of periodontopathogenic bacteria, Prevotella tannerae, Fusobacterium nucleatum, and Prevotella intermedia, were associated with an increased OSCC risk. This association was modified by the genetic polymorphisms of TLR2 and TLR4. Use of alcohol, betel quids and cigarettes and poor oral hygiene were associated with a higher percentage of oral periodontopathogenic bacteria. The association between alcohol and periodontopathogenic bacteria was modified by the genetic polymorphism of ALDH2, with a stronger positive association observed among the ALDH2-deficient individuals. The percentage of periodontopathogenic bacteria was positively correlated with the level of salivary IL1β, an inflammatory cytokine. Overall, our results showed a positive association between periodontopathogenic bacteria and OSCC risk and this relationship may be influenced by lifestyle and genetic factors. Our results provided further biological support for the established association between poor oral hygiene and OSCC risk. This suggested that improving oral hygiene may reduce OSCC risk and should be part of a public health campaign to prevent the occurrence of OSCC.",
author = "Jenn-Ren Hsiao and Chan-Chi Chang and Wei-Ting Lee and Cheng-Chih Huang and Chun-Yen Ou and Sen-Tien Tsai and Ken-Chung Chen and Jehn-Shun Huang and Dung-Yau Wang and Yu-Hsuan Lai and Yuan-Hua Wu and Wei-Ting Hsueh and Shang-Yin Wu and Chia-Jui Yen and Jang-Yang Chang and Lin, {Chen Lin} and Weng, {Ya Ling} and Yang, {Han Chien} and Chen, {Yu Shan} and Chang, {Jeffrey S.}",
year = "2018",
month = "5",
day = "28",
doi = "10.1093/carcin/bgy053",
language = "English",
volume = "39",
pages = "778--787",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
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T1 - The interplay between oral microbiome, lifestyle factors and genetic polymorphisms in the risk of oral squamous cell carcinoma

AU - Hsiao, Jenn-Ren

AU - Chang, Chan-Chi

AU - Lee, Wei-Ting

AU - Huang, Cheng-Chih

AU - Ou, Chun-Yen

AU - Tsai, Sen-Tien

AU - Chen, Ken-Chung

AU - Huang, Jehn-Shun

AU - Wang, Dung-Yau

AU - Lai, Yu-Hsuan

AU - Wu, Yuan-Hua

AU - Hsueh, Wei-Ting

AU - Wu, Shang-Yin

AU - Yen, Chia-Jui

AU - Chang, Jang-Yang

AU - Lin, Chen Lin

AU - Weng, Ya Ling

AU - Yang, Han Chien

AU - Chen, Yu Shan

AU - Chang, Jeffrey S.

PY - 2018/5/28

Y1 - 2018/5/28

N2 - Poor oral hygiene may lead to overgrowth of pathogenic oral bacteria, which may induce chronic inflammation to promote the oncogenesis of oral squamous cell carcinoma (OSCC). This study investigated the association between oral bacterial profile and OSCC risk in a case-control study of 138 OSCC cases and 151 controls (88 cases and 90 controls for the discovery group and 50 cases and 61 controls for the validation group). Oral bacterial profiles were characterized by targeted sequencing of the 16S rRNA gene. Three species of periodontopathogenic bacteria, Prevotella tannerae, Fusobacterium nucleatum, and Prevotella intermedia, were associated with an increased OSCC risk. This association was modified by the genetic polymorphisms of TLR2 and TLR4. Use of alcohol, betel quids and cigarettes and poor oral hygiene were associated with a higher percentage of oral periodontopathogenic bacteria. The association between alcohol and periodontopathogenic bacteria was modified by the genetic polymorphism of ALDH2, with a stronger positive association observed among the ALDH2-deficient individuals. The percentage of periodontopathogenic bacteria was positively correlated with the level of salivary IL1β, an inflammatory cytokine. Overall, our results showed a positive association between periodontopathogenic bacteria and OSCC risk and this relationship may be influenced by lifestyle and genetic factors. Our results provided further biological support for the established association between poor oral hygiene and OSCC risk. This suggested that improving oral hygiene may reduce OSCC risk and should be part of a public health campaign to prevent the occurrence of OSCC.

AB - Poor oral hygiene may lead to overgrowth of pathogenic oral bacteria, which may induce chronic inflammation to promote the oncogenesis of oral squamous cell carcinoma (OSCC). This study investigated the association between oral bacterial profile and OSCC risk in a case-control study of 138 OSCC cases and 151 controls (88 cases and 90 controls for the discovery group and 50 cases and 61 controls for the validation group). Oral bacterial profiles were characterized by targeted sequencing of the 16S rRNA gene. Three species of periodontopathogenic bacteria, Prevotella tannerae, Fusobacterium nucleatum, and Prevotella intermedia, were associated with an increased OSCC risk. This association was modified by the genetic polymorphisms of TLR2 and TLR4. Use of alcohol, betel quids and cigarettes and poor oral hygiene were associated with a higher percentage of oral periodontopathogenic bacteria. The association between alcohol and periodontopathogenic bacteria was modified by the genetic polymorphism of ALDH2, with a stronger positive association observed among the ALDH2-deficient individuals. The percentage of periodontopathogenic bacteria was positively correlated with the level of salivary IL1β, an inflammatory cytokine. Overall, our results showed a positive association between periodontopathogenic bacteria and OSCC risk and this relationship may be influenced by lifestyle and genetic factors. Our results provided further biological support for the established association between poor oral hygiene and OSCC risk. This suggested that improving oral hygiene may reduce OSCC risk and should be part of a public health campaign to prevent the occurrence of OSCC.

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U2 - 10.1093/carcin/bgy053

DO - 10.1093/carcin/bgy053

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VL - 39

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JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 6

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