The involvement of metabotropic glutamate receptors in long-term depression of N-methyl-d-aspartate receptor-mediated synaptic potential in the rat hippocampus

Pei Lu Yi, Fang Chia Chang, Jing Jane Tsai, Chen Road Hung, Po-Wu Gean

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Abstract

The frequency-dependent long-term modifications of pharmacologically isolated N-methyl-d-aspartate (NMDA) receptor-mediated excitatory postsynaptic potential (EPSPNMDA) was studied. Intracellular recordings were obtained from CA1 cells of rat hippocampal slices and in the presence of 6-cyano-7-nitroquinoxaline-2,3-dione (10 μM) and bicuculline (20 μM) which block non-NMDA and GABAA receptors, respectively. Low-frequency stimulation at 5 Hz resulted in a long-term depression (LTD) of EPSPNMDA in 12 of 17 cells. However, when the stimulus frequency was increased to 30 Hz, a long-term potentiation (LTP) of EPSPNMDA was observed in 7 out of 9 cells. The LTD was not affected by pretreating the slices with okadaic acid (0.5-1 μM) suggesting that activation of endogenous protein phosphatase is not responsible for this process. In the presence of l-2-amino-3-phosphonopropionic acid (50 μM) or (RS)-α-methyl-4-carboxyphenylglycine (200 μM), 5 Hz tetanization resulted in LTP instead of LTD. These results suggest that activation of metabotropic glutamate receptor (mGluR) is necessary for the induction of EPSPNMDA LTD and blockade of mGluR unmasks a LTP.

Original languageEnglish
Pages (from-to)207-210
Number of pages4
JournalNeuroscience Letters
Volume185
Issue number3
DOIs
Publication statusPublished - 1995 Feb 13

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Synaptic Potentials
Metabotropic Glutamate Receptors
Long-Term Potentiation
Hippocampus
6-Cyano-7-nitroquinoxaline-2,3-dione
Okadaic Acid
Bicuculline
Excitatory Postsynaptic Potentials
Phosphoprotein Phosphatases
GABA-A Receptors
aspartic acid receptor

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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title = "The involvement of metabotropic glutamate receptors in long-term depression of N-methyl-d-aspartate receptor-mediated synaptic potential in the rat hippocampus",
abstract = "The frequency-dependent long-term modifications of pharmacologically isolated N-methyl-d-aspartate (NMDA) receptor-mediated excitatory postsynaptic potential (EPSPNMDA) was studied. Intracellular recordings were obtained from CA1 cells of rat hippocampal slices and in the presence of 6-cyano-7-nitroquinoxaline-2,3-dione (10 μM) and bicuculline (20 μM) which block non-NMDA and GABAA receptors, respectively. Low-frequency stimulation at 5 Hz resulted in a long-term depression (LTD) of EPSPNMDA in 12 of 17 cells. However, when the stimulus frequency was increased to 30 Hz, a long-term potentiation (LTP) of EPSPNMDA was observed in 7 out of 9 cells. The LTD was not affected by pretreating the slices with okadaic acid (0.5-1 μM) suggesting that activation of endogenous protein phosphatase is not responsible for this process. In the presence of l-2-amino-3-phosphonopropionic acid (50 μM) or (RS)-α-methyl-4-carboxyphenylglycine (200 μM), 5 Hz tetanization resulted in LTP instead of LTD. These results suggest that activation of metabotropic glutamate receptor (mGluR) is necessary for the induction of EPSPNMDA LTD and blockade of mGluR unmasks a LTP.",
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The involvement of metabotropic glutamate receptors in long-term depression of N-methyl-d-aspartate receptor-mediated synaptic potential in the rat hippocampus. / Yi, Pei Lu; Chang, Fang Chia; Tsai, Jing Jane; Hung, Chen Road; Gean, Po-Wu.

In: Neuroscience Letters, Vol. 185, No. 3, 13.02.1995, p. 207-210.

Research output: Contribution to journalArticle

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