The Kindlins: Subcellular localization and expression during murine development

Siegfried Ussar, Hao Ven Wang, Stefan Linder, Reinhard Fässler, Markus Moser

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

The three Kindlins are a novel family of focal adhesion proteins. The Kindlin-1 (URP1) gene is mutated in Kindler syndrome, the first skin blistering disease affecting actin attachment in basal keratinocytes. Kindlin-2 (Mig-2), the best studied member of this family, binds ILK and Migfilin, which links Kindlin-2 to the actin cytoskeleton. Kindlin-3 is expressed in hematopoietic cells. Here we describe the genomic organization, gene expression and subcellular localization of murine Kindlins-1 to -3. In situ hybridizations showed that Kindlin-1 is preferentially expressed in epithelia, and Kindlin-2 in striated and smooth muscle cells. Kindlins-1 and -2 are both expressed in the epidermis. While both localize to integrin-mediated adhesion sites in cultured keratinocytes Kindlin-2, but not Kindlin-1, colocalizes with E-cadherin to cell-cell contacts in differentiated keratinocytes. Using a Kindlin-3-specific antiserum and an EGFP-tagged Kindlin-3 construct, we could show that Kindlin-3 is present in the F-actin surrounding ring structure of podosomes, which are specialized adhesion structures of hematopoietic cells.

Original languageEnglish
Pages (from-to)3142-3151
Number of pages10
JournalExperimental Cell Research
Volume312
Issue number16
DOIs
Publication statusPublished - 2006 Oct 1

All Science Journal Classification (ASJC) codes

  • Cell Biology

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