The mechanism of inhibitory actions of S-petasin, a sesquiterpene of Petasites formosanus, on L-type calcium current in NG108-15 neuronal cells

Sheng-Nan Wu, Hsinyo Chen, Yun Lian Lin

Research output: Contribution to journalArticle

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Abstract

The effects of S-petasin, a sesquiterpene isolated from Petasites formosanus Kitamura, on ion currents in a mouse neuroblastoma and a rat glioma hybrid cell line, NG108-15, were examined with the aid of the whole-cell voltage-clamp technique. S-Petasin (1-300 μM) caused a decrease in the amplitude of L-type Ca 2+ current (I Ca,L ) in a concentration-dependent manner, however, it did not change the overall shape of the current-voltage relationship of I Ca,L . The IC 50 value for S-petasin-induced inhibition of I Ca,L was 11 μM. S-Petasin (10 μM) shifted the steady-state inactivation of I Ca,L to a more negative membrane potential by approximately -10 mV. S-petasin could prolong the recovery of I Ca,L inactivation. The inhibitory effect of S-petasin on I Ca,L was found to exhibit tonic and use-dependent characteristics. S-Petasin could inhibit I Ca,L evoked by action potential waveforms effectively. S-Petasin also suppressed low voltage-activated I Ca,L in NG108-15 cells. S-Petasin at a concentration of 100 μM had little effect on voltage-dependent Na + current; however, it did produce an inhibitory effect on delayed rectifier K + current in a time-dependent manner. These results demonstrate that S-petasin can interact directly with L-type Ca 2+ channels in NG108-15 cells. These effects could contribute to the regulation of neuronal activity if similar results were found in neurons in vivo.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalPlanta Medica
Volume69
Issue number2
DOIs
Publication statusPublished - 2003 Feb 1

Fingerprint

Petasites
Sesquiterpenes
Calcium
Electric potential
petasin
Hybrid Cells
Clamping devices
Patch-Clamp Techniques
Neuroblastoma
Evoked Potentials
Glioma
Membrane Potentials
Action Potentials
Neurons
Rats

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this

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abstract = "The effects of S-petasin, a sesquiterpene isolated from Petasites formosanus Kitamura, on ion currents in a mouse neuroblastoma and a rat glioma hybrid cell line, NG108-15, were examined with the aid of the whole-cell voltage-clamp technique. S-Petasin (1-300 μM) caused a decrease in the amplitude of L-type Ca 2+ current (I Ca,L ) in a concentration-dependent manner, however, it did not change the overall shape of the current-voltage relationship of I Ca,L . The IC 50 value for S-petasin-induced inhibition of I Ca,L was 11 μM. S-Petasin (10 μM) shifted the steady-state inactivation of I Ca,L to a more negative membrane potential by approximately -10 mV. S-petasin could prolong the recovery of I Ca,L inactivation. The inhibitory effect of S-petasin on I Ca,L was found to exhibit tonic and use-dependent characteristics. S-Petasin could inhibit I Ca,L evoked by action potential waveforms effectively. S-Petasin also suppressed low voltage-activated I Ca,L in NG108-15 cells. S-Petasin at a concentration of 100 μM had little effect on voltage-dependent Na + current; however, it did produce an inhibitory effect on delayed rectifier K + current in a time-dependent manner. These results demonstrate that S-petasin can interact directly with L-type Ca 2+ channels in NG108-15 cells. These effects could contribute to the regulation of neuronal activity if similar results were found in neurons in vivo.",
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The mechanism of inhibitory actions of S-petasin, a sesquiterpene of Petasites formosanus, on L-type calcium current in NG108-15 neuronal cells. / Wu, Sheng-Nan; Chen, Hsinyo; Lin, Yun Lian.

In: Planta Medica, Vol. 69, No. 2, 01.02.2003, p. 118-124.

Research output: Contribution to journalArticle

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AU - Wu, Sheng-Nan

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AB - The effects of S-petasin, a sesquiterpene isolated from Petasites formosanus Kitamura, on ion currents in a mouse neuroblastoma and a rat glioma hybrid cell line, NG108-15, were examined with the aid of the whole-cell voltage-clamp technique. S-Petasin (1-300 μM) caused a decrease in the amplitude of L-type Ca 2+ current (I Ca,L ) in a concentration-dependent manner, however, it did not change the overall shape of the current-voltage relationship of I Ca,L . The IC 50 value for S-petasin-induced inhibition of I Ca,L was 11 μM. S-Petasin (10 μM) shifted the steady-state inactivation of I Ca,L to a more negative membrane potential by approximately -10 mV. S-petasin could prolong the recovery of I Ca,L inactivation. The inhibitory effect of S-petasin on I Ca,L was found to exhibit tonic and use-dependent characteristics. S-Petasin could inhibit I Ca,L evoked by action potential waveforms effectively. S-Petasin also suppressed low voltage-activated I Ca,L in NG108-15 cells. S-Petasin at a concentration of 100 μM had little effect on voltage-dependent Na + current; however, it did produce an inhibitory effect on delayed rectifier K + current in a time-dependent manner. These results demonstrate that S-petasin can interact directly with L-type Ca 2+ channels in NG108-15 cells. These effects could contribute to the regulation of neuronal activity if similar results were found in neurons in vivo.

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