The mitochondrial dna markers for distinguishing phalaenopsis species and revealing maternal phylogeny

Ching Chun Chang, Bo Yen Lin, Yu Chang Liu, Ting Chieh Chen, Yu Ya Su, Wen-Luan Wu

Research output: Chapter in Book/Report/Conference proceedingChapter


We explored the mitochondrial DNA (mtDNA) markers for species identification via located simple sequence repeats in the mtDNA of Phalaenopsis aphrodite subsp. formosana, and then pre-screened for polymorphic markers by comparison with the corresponding mtDNA regions of P. equestris. The combination of 13 selected markers located in intergenic spacers could unambiguously distinguish the 15 endemic moth orchids. The five most variable markers with polymorphic information content (PIC) ≥ 0.7, could be combined to classify 18 of the 19 endemic moth orchids, including the parental strains most commonly used in breeding programs. The sequences of four selected mtDNA regions were highly variable, and one region (MT2) could be used to completely distinguish 19 endemic moth orchids. Though mitochondrial introns were highly conserved among moth orchids; evolutionary hotspots such as variable SSRs and minisatellite repeats were identified as useful markers. Furthermore, marker technology was applied to reveal the maternal inheritance mode of mtDNA in moth orchids. Moreover, phylogenetic analysis indicates that mtDNA is non-monophyletic below Phalaenopsis genus. In summary, we have revealed a set of mtDNA markers that could be used for identification and phylogenetic study of Phalaenopsis orchids.

Original languageEnglish
Title of host publicationOrchid Biotechnology Iv
PublisherWorld Scientific Publishing Co. Pte Ltd
Number of pages22
ISBN (Electronic)9789811217777
ISBN (Print)9789811217760
Publication statusPublished - 2021 Mar 23

All Science Journal Classification (ASJC) codes

  • General Agricultural and Biological Sciences
  • General Biochemistry,Genetics and Molecular Biology


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