The mus206 gene of Drosophila melanogaster is required in the excision repair of alkylation-induced DNA lesions

Wenya Huang, P. Dennis Smith

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

The mus206(A1) mutation, previously identified in our laboratory on the basis of increased sensitivity to methyl methanesulfonate (MMS), has undergone further analysis. Genetic recombinational mapping data localize mus206 at 2-54.8. Sex-linked recessive lethal mutation tests indicate that mus206(A1) exhibits significant alkylation-induced hypermutability, compared to the wild-type Oregon R progenitor strain, suggesting a defect in DNA repair function. Results of embryo viability tests show that mus206(A1) and Oregon R embryos hatch to the first instar larvae at similar rates, indicating that the mus206(A1) mutation does not confer embryonic lethality. Unscheduled DNA synthesis (UDS) studies with primary embryonic cell cultures subsequently demonstrated considerably less nucleotide incorporation following treatment with MMS, confirming that mus206(A1) is deficient at or before the resynthesis step of alkylation-induced DNA excision repair. Previous genetic investigations have provided indirect support that at least 15 Drosophila genes which display MMS sensitivity are deficient in DNA repair functions. This study brings to 7 the number of mus genes displaying alkylation excision-repair deficiency.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalMutation Research - DNA Repair
Volume384
Issue number2
DOIs
Publication statusPublished - 1997 Aug

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Toxicology
  • Genetics

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