Abstract
Nucleotide excision repair (NER) of DNA in the yeast Saccharomyces cerevisiae and in human cells has been shown to be a biochemically complex process involving multiple gene products. In yeast, the involvement of the DNA replication accessory proteins, replication protein A (RPA1) and proliferating cell nuclear antigen (PCNA) in NER has not been demonstrated genetically. In this study we have generated temperature-degradable rfa1 and pcna mutants and show that these mutants are defective in NER in vitro under conditions that promote degradation of the RFA1 and PCNA gene products. We also demonstrate a physical interaction between RPA1 protein and subunits of the RNA polymerase II basal transcription factor IIH (TFIIH). Copyright (C) 1998 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 183-194 |
| Number of pages | 12 |
| Journal | Mutation Research - DNA Repair |
| Volume | 408 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1998 Sep 11 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Toxicology
- Genetics
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The N-degron protein degradation strategy for investigating the function of essential genes : requirement for replication protein A and proliferating cell nuclear antigen proteins for nucleotide excision repair in yeast extracts. / Huang, Wen-Ya; Feaver, William J.; Tomkinson, Alan E.; Friedberg, Errol C.
In: Mutation Research - DNA Repair, Vol. 408, No. 3, 11.09.1998, p. 183-194.Research output: Contribution to journal › Article
TY - JOUR
T1 - The N-degron protein degradation strategy for investigating the function of essential genes
T2 - requirement for replication protein A and proliferating cell nuclear antigen proteins for nucleotide excision repair in yeast extracts
AU - Huang, Wen-Ya
AU - Feaver, William J.
AU - Tomkinson, Alan E.
AU - Friedberg, Errol C.
PY - 1998/9/11
Y1 - 1998/9/11
N2 - Nucleotide excision repair (NER) of DNA in the yeast Saccharomyces cerevisiae and in human cells has been shown to be a biochemically complex process involving multiple gene products. In yeast, the involvement of the DNA replication accessory proteins, replication protein A (RPA1) and proliferating cell nuclear antigen (PCNA) in NER has not been demonstrated genetically. In this study we have generated temperature-degradable rfa1 and pcna mutants and show that these mutants are defective in NER in vitro under conditions that promote degradation of the RFA1 and PCNA gene products. We also demonstrate a physical interaction between RPA1 protein and subunits of the RNA polymerase II basal transcription factor IIH (TFIIH). Copyright (C) 1998 Elsevier Science B.V.
AB - Nucleotide excision repair (NER) of DNA in the yeast Saccharomyces cerevisiae and in human cells has been shown to be a biochemically complex process involving multiple gene products. In yeast, the involvement of the DNA replication accessory proteins, replication protein A (RPA1) and proliferating cell nuclear antigen (PCNA) in NER has not been demonstrated genetically. In this study we have generated temperature-degradable rfa1 and pcna mutants and show that these mutants are defective in NER in vitro under conditions that promote degradation of the RFA1 and PCNA gene products. We also demonstrate a physical interaction between RPA1 protein and subunits of the RNA polymerase II basal transcription factor IIH (TFIIH). Copyright (C) 1998 Elsevier Science B.V.
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U2 - 10.1016/S0921-8777(98)00031-7
DO - 10.1016/S0921-8777(98)00031-7
M3 - Article
VL - 408
SP - 183
EP - 194
JO - Mutation Research - DNA Repair
JF - Mutation Research - DNA Repair
SN - 0921-8777
IS - 3
ER -