TY - JOUR
T1 - The N-degron protein degradation strategy for investigating the function of essential genes
T2 - requirement for replication protein A and proliferating cell nuclear antigen proteins for nucleotide excision repair in yeast extracts
AU - Huang, Wenya
AU - Feaver, William J.
AU - Tomkinson, Alan E.
AU - Friedberg, Errol C.
N1 - Funding Information:
We thank Paul Berg, Peter Burgers, Richard Kolodner and Paulo Plavani for various rfa1 and pcna mutant strains, Gerhard Cullman and Bruce Stillman for purified recombinant PCNA protein and Steven Brill for antisera against RPA1 and RPA2 proteins. We also acknowledge our laboratory colleagues for helpful comments and suggestions. These studies were supported by research grant CA12420 from the United States Public Health Service (ECF) and grant 3786 from the Council for Tobacco Research (AET). WJF is a Fellow of the Jane Coffin Childs Memorial Fund for Medical Research.
PY - 1998/9/11
Y1 - 1998/9/11
N2 - Nucleotide excision repair (NER) of DNA in the yeast Saccharomyces cerevisiae and in human cells has been shown to be a biochemically complex process involving multiple gene products. In yeast, the involvement of the DNA replication accessory proteins, replication protein A (RPA1) and proliferating cell nuclear antigen (PCNA) in NER has not been demonstrated genetically. In this study we have generated temperature-degradable rfa1 and pcna mutants and show that these mutants are defective in NER in vitro under conditions that promote degradation of the RFA1 and PCNA gene products. We also demonstrate a physical interaction between RPA1 protein and subunits of the RNA polymerase II basal transcription factor IIH (TFIIH). Copyright (C) 1998 Elsevier Science B.V.
AB - Nucleotide excision repair (NER) of DNA in the yeast Saccharomyces cerevisiae and in human cells has been shown to be a biochemically complex process involving multiple gene products. In yeast, the involvement of the DNA replication accessory proteins, replication protein A (RPA1) and proliferating cell nuclear antigen (PCNA) in NER has not been demonstrated genetically. In this study we have generated temperature-degradable rfa1 and pcna mutants and show that these mutants are defective in NER in vitro under conditions that promote degradation of the RFA1 and PCNA gene products. We also demonstrate a physical interaction between RPA1 protein and subunits of the RNA polymerase II basal transcription factor IIH (TFIIH). Copyright (C) 1998 Elsevier Science B.V.
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U2 - 10.1016/S0921-8777(98)00031-7
DO - 10.1016/S0921-8777(98)00031-7
M3 - Article
C2 - 9806417
AN - SCOPUS:0031720580
VL - 408
SP - 183
EP - 194
JO - Mutation Research - DNA Repair
JF - Mutation Research - DNA Repair
SN - 0921-8777
IS - 3
ER -