The negative effects of KPN00353 on glycerol kinase and microaerobic 1,3-propanediol production in klebsiella pneumoniae

Wen-Yih Jeng, Novaria S.D. Panjaitan, Yu Tze Horng, Wen Ting Chung, Chih Ching Chien, Po Chi Soo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

1,3-Propanediol (1,3-PD) is a valuable chemical intermediate in the synthesis of polyesters, polyethers, and polyurethanes, which have applications in various products such as cloth, bottles, films, tarpaulins, canoes, foam seals, high-resilience foam seating, and surface coatings. Klebsiella pneumoniae can produce 1,3-PD from glycerol. In this study, KPN00353, an EIIA homologue in the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS), was found to play a negative regulatory role in 1,3-PD production under microaerobic conditions via binding to glycerol kinase (GlpK). The primary sequence of KPN00353 is similar to those of the fructose-mannitol EIIA (EIIFru and EIIAMtl) family. The interaction between KPN00353 and GlpK resulted in inhibition of the synthesis of glycerol-3-phosphate (G3P) and correlated with reductions in glycerol uptake and the production of 1,3-PD. Based on structure modeling, we conclude that residue H65 of KPN00353 plays an important role in the interaction with GlpK. We mutated this histidine residue to aspartate, glutamate, arginine and glutamine to assess the effects of each KPN00353 variant on the interaction with GlpK, on the synthesis of G3P and on the production of 1,3-PD. Our results illuminate the role of KPN00353 in 1,3-PD production by K. pneumoniae under microaerobic conditions.

Original languageEnglish
Article number2441
JournalFrontiers in Microbiology
Volume8
Issue numberDEC
DOIs
Publication statusPublished - 2017 Dec 7

Fingerprint

Glycerol Kinase
Klebsiella pneumoniae
arginine glutamate
Glycerol
Phosphoenolpyruvate
Polyesters
Polyurethanes
Mannitol
Fructose
Glutamine
Histidine
1,3-propanediol
Phosphotransferases
Carbohydrates

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Jeng, Wen-Yih ; Panjaitan, Novaria S.D. ; Horng, Yu Tze ; Chung, Wen Ting ; Chien, Chih Ching ; Soo, Po Chi. / The negative effects of KPN00353 on glycerol kinase and microaerobic 1,3-propanediol production in klebsiella pneumoniae. In: Frontiers in Microbiology. 2017 ; Vol. 8, No. DEC.
@article{afcc03f0580e406f8ac134926171c82d,
title = "The negative effects of KPN00353 on glycerol kinase and microaerobic 1,3-propanediol production in klebsiella pneumoniae",
abstract = "1,3-Propanediol (1,3-PD) is a valuable chemical intermediate in the synthesis of polyesters, polyethers, and polyurethanes, which have applications in various products such as cloth, bottles, films, tarpaulins, canoes, foam seals, high-resilience foam seating, and surface coatings. Klebsiella pneumoniae can produce 1,3-PD from glycerol. In this study, KPN00353, an EIIA homologue in the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS), was found to play a negative regulatory role in 1,3-PD production under microaerobic conditions via binding to glycerol kinase (GlpK). The primary sequence of KPN00353 is similar to those of the fructose-mannitol EIIA (EIIFru and EIIAMtl) family. The interaction between KPN00353 and GlpK resulted in inhibition of the synthesis of glycerol-3-phosphate (G3P) and correlated with reductions in glycerol uptake and the production of 1,3-PD. Based on structure modeling, we conclude that residue H65 of KPN00353 plays an important role in the interaction with GlpK. We mutated this histidine residue to aspartate, glutamate, arginine and glutamine to assess the effects of each KPN00353 variant on the interaction with GlpK, on the synthesis of G3P and on the production of 1,3-PD. Our results illuminate the role of KPN00353 in 1,3-PD production by K. pneumoniae under microaerobic conditions.",
author = "Wen-Yih Jeng and Panjaitan, {Novaria S.D.} and Horng, {Yu Tze} and Chung, {Wen Ting} and Chien, {Chih Ching} and Soo, {Po Chi}",
year = "2017",
month = "12",
day = "7",
doi = "10.3389/fmicb.2017.02441",
language = "English",
volume = "8",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S. A.",
number = "DEC",

}

The negative effects of KPN00353 on glycerol kinase and microaerobic 1,3-propanediol production in klebsiella pneumoniae. / Jeng, Wen-Yih; Panjaitan, Novaria S.D.; Horng, Yu Tze; Chung, Wen Ting; Chien, Chih Ching; Soo, Po Chi.

In: Frontiers in Microbiology, Vol. 8, No. DEC, 2441, 07.12.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The negative effects of KPN00353 on glycerol kinase and microaerobic 1,3-propanediol production in klebsiella pneumoniae

AU - Jeng, Wen-Yih

AU - Panjaitan, Novaria S.D.

AU - Horng, Yu Tze

AU - Chung, Wen Ting

AU - Chien, Chih Ching

AU - Soo, Po Chi

PY - 2017/12/7

Y1 - 2017/12/7

N2 - 1,3-Propanediol (1,3-PD) is a valuable chemical intermediate in the synthesis of polyesters, polyethers, and polyurethanes, which have applications in various products such as cloth, bottles, films, tarpaulins, canoes, foam seals, high-resilience foam seating, and surface coatings. Klebsiella pneumoniae can produce 1,3-PD from glycerol. In this study, KPN00353, an EIIA homologue in the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS), was found to play a negative regulatory role in 1,3-PD production under microaerobic conditions via binding to glycerol kinase (GlpK). The primary sequence of KPN00353 is similar to those of the fructose-mannitol EIIA (EIIFru and EIIAMtl) family. The interaction between KPN00353 and GlpK resulted in inhibition of the synthesis of glycerol-3-phosphate (G3P) and correlated with reductions in glycerol uptake and the production of 1,3-PD. Based on structure modeling, we conclude that residue H65 of KPN00353 plays an important role in the interaction with GlpK. We mutated this histidine residue to aspartate, glutamate, arginine and glutamine to assess the effects of each KPN00353 variant on the interaction with GlpK, on the synthesis of G3P and on the production of 1,3-PD. Our results illuminate the role of KPN00353 in 1,3-PD production by K. pneumoniae under microaerobic conditions.

AB - 1,3-Propanediol (1,3-PD) is a valuable chemical intermediate in the synthesis of polyesters, polyethers, and polyurethanes, which have applications in various products such as cloth, bottles, films, tarpaulins, canoes, foam seals, high-resilience foam seating, and surface coatings. Klebsiella pneumoniae can produce 1,3-PD from glycerol. In this study, KPN00353, an EIIA homologue in the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS), was found to play a negative regulatory role in 1,3-PD production under microaerobic conditions via binding to glycerol kinase (GlpK). The primary sequence of KPN00353 is similar to those of the fructose-mannitol EIIA (EIIFru and EIIAMtl) family. The interaction between KPN00353 and GlpK resulted in inhibition of the synthesis of glycerol-3-phosphate (G3P) and correlated with reductions in glycerol uptake and the production of 1,3-PD. Based on structure modeling, we conclude that residue H65 of KPN00353 plays an important role in the interaction with GlpK. We mutated this histidine residue to aspartate, glutamate, arginine and glutamine to assess the effects of each KPN00353 variant on the interaction with GlpK, on the synthesis of G3P and on the production of 1,3-PD. Our results illuminate the role of KPN00353 in 1,3-PD production by K. pneumoniae under microaerobic conditions.

UR - http://www.scopus.com/inward/record.url?scp=85037345601&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85037345601&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2017.02441

DO - 10.3389/fmicb.2017.02441

M3 - Article

AN - SCOPUS:85037345601

VL - 8

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

IS - DEC

M1 - 2441

ER -