The novel organization and complete sequence of the ribosomal RNA gene of Nosema bombycis

Wei Fone Huang, Shu Jen Tsai, Chu-Fang Lo, Yamane Soichi, Chung Hsiung Wang

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)

Abstract

We present here for the first time the complete DNA sequence data (4301bp) of the ribosomal RNA (rRNA) gene of the microsporidian type species, Nosema bombycis. Sequences for the large subunit gene (LSUrRNA: 2497bp, GenBank Accession No. AY211393), the internal transcribed spacer (ITS: 179bp, GenBank Accession No. AY211394), the small subunit gene (SSUrRNA: 1232bp), intergenic spacer (IGS: 279bp), and 5S region (114bp) are also given, and the secondary structure of the large subunit is discussed. The organization of the N. bombycis rRNA gene is LSUrRNA-ITS-SSUrRNA-IGS-5S. This novel arrangement, in which the LSU is 5 of the SSU, is the reverse of the organizational sequence (i.e., SSU-ITS-LSU) found in all previously reported microsporidian rRNAs, including Nosema apis. This unique character in the type species may have taxonomic implications for the members of the genus Nosema. Index descriptors: Nosema; rRNA organization; microsporidia

Original languageEnglish
Pages (from-to)473-481
Number of pages9
JournalFungal Genetics and Biology
Volume41
Issue number5
DOIs
Publication statusPublished - 2004 May 1

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics

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