The optimal vibrational shear stress for bovine endothelial cell proliferation

Ching Wen Li, Jau Liang Chen, Chia-Ching Wu, Gou Jen Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, a novel method for quantitative analysis of the optimal shear stress for better cell growth using a micro-positioning piezoelectric lead zirconate titanate (PZT) stage is proposed. Compared with the conventional fluidic sheer stress, a micro- positioning PZT stage not only provides cultured cells with laminar flow, but also is able to generate large shear stress gradient by precise reciprocating motions. Bovine endothelial cells (BEC) are than cultured on different scaffolds for investigation of cell proliferation under different vibrational excitations by a PZT stage. The fluorescence labeling is adopted to estimate the adhesive area of a cultured cell. It is observed that cells cultured on different artificial scaffolds adjusted their adhesion area to respond to the shear stress induced stimulus. Optimal growth curves for BECs on different scaffolds are drawn. The optimal shear stress for the proliferation of BECs on different scaffolds is found to be closely identical. It is suggested that a micro-positioning PZT stage may be a more cost and time effective solution than the nanostructured scaffold approach for the enhancement of cell growth.

Original languageEnglish
Title of host publication2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Pages716-720
Number of pages5
DOIs
Publication statusPublished - 2012 Jun 1
Event7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 - Kyoto, Japan
Duration: 2012 Mar 52012 Mar 8

Publication series

Name2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

Other

Other7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
CountryJapan
CityKyoto
Period12-03-0512-03-08

Fingerprint

Endothelial cells
Cell proliferation
Shear stress
Lead
Scaffolds
Cell growth
Scaffolds (biology)
Cells
Fluidics
Laminar flow
Labeling
Adhesives
Adhesion
Fluorescence
Chemical analysis
Costs

All Science Journal Classification (ASJC) codes

  • Engineering (miscellaneous)

Cite this

Li, C. W., Chen, J. L., Wu, C-C., & Wang, G. J. (2012). The optimal vibrational shear stress for bovine endothelial cell proliferation. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 (pp. 716-720). [6196875] (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). https://doi.org/10.1109/NEMS.2012.6196875
Li, Ching Wen ; Chen, Jau Liang ; Wu, Chia-Ching ; Wang, Gou Jen. / The optimal vibrational shear stress for bovine endothelial cell proliferation. 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. pp. 716-720 (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012).
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Li, CW, Chen, JL, Wu, C-C & Wang, GJ 2012, The optimal vibrational shear stress for bovine endothelial cell proliferation. in 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012., 6196875, 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012, pp. 716-720, 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012, Kyoto, Japan, 12-03-05. https://doi.org/10.1109/NEMS.2012.6196875

The optimal vibrational shear stress for bovine endothelial cell proliferation. / Li, Ching Wen; Chen, Jau Liang; Wu, Chia-Ching; Wang, Gou Jen.

2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. p. 716-720 6196875 (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Li CW, Chen JL, Wu C-C, Wang GJ. The optimal vibrational shear stress for bovine endothelial cell proliferation. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012. 2012. p. 716-720. 6196875. (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). https://doi.org/10.1109/NEMS.2012.6196875