The potassium-chloride cotransporter 2 promotes cervical cancer cell migration and invasion by an ion transport-independent mechanism

Wei Chun Wei, Colin J. Akerman, Sarah E. Newey, Jiliu Pan, Nicholas W.V. Clinch, Yves Jacob, Meng-Ru Shen, Robert J. Wilkins, J. Clive Ellory

Research output: Contribution to journalArticle

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Abstract

K +-Cl - cotransporters (KCCs) play a fundamental role in epithelial cell function, both in the context of ionic homeostasis and also in cell morphology, cell division and locomotion. Unlike other ubiquitously expressed KCC isoforms, expression of KCC2 is widely considered to be restricted to neurons, where it is responsible for maintaining a low intracellular chloride concentration to drive hyperpolarising postsynaptic responses to the inhibitory neurotransmitters GABA and glycine. Here we report a novel finding that KCC2 is widely expressed in several human cancer cell lines including the cervical cancer cell line (SiHa). Membrane biotinylation assays and immunostaining showed that endogenous KCC2 is located on the cell membrane of SiHa cells. To elucidate the role of KCC2 in cervical tumuorigenesis, SiHa cells with stable overexpression or knockdown of KCC2 were employed. Overexpression of KCC2 had no significant effect on cell proliferation but dramatically suppressed cell spreading and stress fibre organization, while knockdown of KCC2 showed opposite effects. In addition, insulin-like growth factor 1 (IGF-1)-induced cell migration and invasiveness were significantly increased by overexpression of KCC2. KCC2-induced cell migration and invasion were not dependent on KCC2 transport function since overexpression of an activity-deficient mutant KCC2 still increased IGF-1-induced cell migration and invasion. Moreover, overexpression of KCC2 significantly diminished the number of focal adhesions, while knockdown of KCC2 increased their number. Taken together, our data establish that KCC2 expression and function are not restricted to neurons and that KCC2 serves to increase cervical tumourigenesis via an ion transport-independent mechanism.

Original languageEnglish
Pages (from-to)5349-5359
Number of pages11
JournalJournal of Physiology
Volume589
Issue number22
DOIs
Publication statusPublished - 2011 Nov 1

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Ion Transport
Uterine Cervical Neoplasms
Cell Movement
Somatomedins
Biotinylation
Neurons
Cell Line
Stress Fibers
Focal Adhesions
Cell Division
Glycine
gamma-Aminobutyric Acid
Neurotransmitter Agents
Chlorides
Protein Isoforms
Homeostasis
Epithelial Cells
Cell Proliferation
Cell Membrane
Membranes

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Wei, Wei Chun ; Akerman, Colin J. ; Newey, Sarah E. ; Pan, Jiliu ; Clinch, Nicholas W.V. ; Jacob, Yves ; Shen, Meng-Ru ; Wilkins, Robert J. ; Ellory, J. Clive. / The potassium-chloride cotransporter 2 promotes cervical cancer cell migration and invasion by an ion transport-independent mechanism. In: Journal of Physiology. 2011 ; Vol. 589, No. 22. pp. 5349-5359.
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The potassium-chloride cotransporter 2 promotes cervical cancer cell migration and invasion by an ion transport-independent mechanism. / Wei, Wei Chun; Akerman, Colin J.; Newey, Sarah E.; Pan, Jiliu; Clinch, Nicholas W.V.; Jacob, Yves; Shen, Meng-Ru; Wilkins, Robert J.; Ellory, J. Clive.

In: Journal of Physiology, Vol. 589, No. 22, 01.11.2011, p. 5349-5359.

Research output: Contribution to journalArticle

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AU - Wei, Wei Chun

AU - Akerman, Colin J.

AU - Newey, Sarah E.

AU - Pan, Jiliu

AU - Clinch, Nicholas W.V.

AU - Jacob, Yves

AU - Shen, Meng-Ru

AU - Wilkins, Robert J.

AU - Ellory, J. Clive

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