The putative invertebrate adaptive immune protein Litopenaeus vannamei Dscam (LvDscam) is the first reported Dscam to lack a transmembrane domain and cytoplasmic tail

Pin Hsiang Chou, Hao Shuo Chang, I. Tung Chen, Han You Lin, Yi Min Chen, Huey Lang Yang, K. C.Han Ching Wang

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

It has recently been suggested that Dscam (Down syndrome cell adhesion molecule), a member of the immunoglobulin superfamily (IgSF), plays an essential role in the alternative adaptive immune system of invertebrates. Here, we isolated and characterized the first shrimp Dscam from Litopenaeus vannamei. The LvDscam protein had an extracellular domain but lacked the expected transmembrane domain and cytoplasmic tail, both of which are found in all other members of the Dscam family (and may also be found in other L. vannamei Dscams that have not yet been isolated). In nervous tissue, expression levels of LvDscam were unexpectedly low. Phylogenetic analysis suggests that LvDscam is far from the Dscams found in other invertebrates. Nevertheless, the domain architecture of the extracellular region of LvDscam is similar to other invertebrate Dscams, and it exhibits the typical configuration of 10 immunoglobulin (Ig) domains, 6 fibronectin type 3 domains (FNIII) and one cell attachment sequence (RGD). Cloning and characterization of a total of 62 cDNAs from hemocytes collected from WSSV-free, WSSV-persistent and WSSV-acute-infected shrimp revealed 23 alternative amino acid sequences in the N-terminal of Ig2, 30 in the N-terminal of Ig3 and 13 in the Ig7 domain. This implies that LvDscam can potentially encode at least 8970 unique isoforms. Further analysis suggested that the LvDscam Ig2 and Ig3 regions are more functionally important than Ig7 in the shrimp's specific immune response against WSSV. We discuss how this tail-less, soluble Dscam can still play an active role in alternative adaptive immune response even while its axonal guidance functionality may be impaired.

Original languageEnglish
Pages (from-to)1258-1267
Number of pages10
JournalDevelopmental and Comparative Immunology
Volume33
Issue number12
DOIs
Publication statusPublished - 2009 Dec 1

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Cell Adhesion Molecules
Invertebrates
Down Syndrome
Tail
Proteins
Nerve Tissue
Hemocytes
Adaptive Immunity
Immunoglobulins
Organism Cloning
Amino Acid Sequence
Immune System
Protein Isoforms
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

Cite this

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title = "The putative invertebrate adaptive immune protein Litopenaeus vannamei Dscam (LvDscam) is the first reported Dscam to lack a transmembrane domain and cytoplasmic tail",
abstract = "It has recently been suggested that Dscam (Down syndrome cell adhesion molecule), a member of the immunoglobulin superfamily (IgSF), plays an essential role in the alternative adaptive immune system of invertebrates. Here, we isolated and characterized the first shrimp Dscam from Litopenaeus vannamei. The LvDscam protein had an extracellular domain but lacked the expected transmembrane domain and cytoplasmic tail, both of which are found in all other members of the Dscam family (and may also be found in other L. vannamei Dscams that have not yet been isolated). In nervous tissue, expression levels of LvDscam were unexpectedly low. Phylogenetic analysis suggests that LvDscam is far from the Dscams found in other invertebrates. Nevertheless, the domain architecture of the extracellular region of LvDscam is similar to other invertebrate Dscams, and it exhibits the typical configuration of 10 immunoglobulin (Ig) domains, 6 fibronectin type 3 domains (FNIII) and one cell attachment sequence (RGD). Cloning and characterization of a total of 62 cDNAs from hemocytes collected from WSSV-free, WSSV-persistent and WSSV-acute-infected shrimp revealed 23 alternative amino acid sequences in the N-terminal of Ig2, 30 in the N-terminal of Ig3 and 13 in the Ig7 domain. This implies that LvDscam can potentially encode at least 8970 unique isoforms. Further analysis suggested that the LvDscam Ig2 and Ig3 regions are more functionally important than Ig7 in the shrimp's specific immune response against WSSV. We discuss how this tail-less, soluble Dscam can still play an active role in alternative adaptive immune response even while its axonal guidance functionality may be impaired.",
author = "Chou, {Pin Hsiang} and Chang, {Hao Shuo} and Chen, {I. Tung} and Lin, {Han You} and Chen, {Yi Min} and Yang, {Huey Lang} and Wang, {K. C.Han Ching}",
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The putative invertebrate adaptive immune protein Litopenaeus vannamei Dscam (LvDscam) is the first reported Dscam to lack a transmembrane domain and cytoplasmic tail. / Chou, Pin Hsiang; Chang, Hao Shuo; Chen, I. Tung; Lin, Han You; Chen, Yi Min; Yang, Huey Lang; Wang, K. C.Han Ching.

In: Developmental and Comparative Immunology, Vol. 33, No. 12, 01.12.2009, p. 1258-1267.

Research output: Contribution to journalArticle

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T1 - The putative invertebrate adaptive immune protein Litopenaeus vannamei Dscam (LvDscam) is the first reported Dscam to lack a transmembrane domain and cytoplasmic tail

AU - Chou, Pin Hsiang

AU - Chang, Hao Shuo

AU - Chen, I. Tung

AU - Lin, Han You

AU - Chen, Yi Min

AU - Yang, Huey Lang

AU - Wang, K. C.Han Ching

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