Abstract
FMN riboswitches are genetic elements that, in many bacteria, control genes responsible for biosynthesis and/or transport of riboflavin (vitamin B2). We report that the Escherichia coli ribB FMN riboswitch controls expression of the essential gene ribB coding for the riboflavin biosynthetic enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase (RibB; EC 4.1.99.12). Our data show that the E. coli ribB FMN riboswitch is unusual because it operates at the transcriptional and also at the translational level. Expression of ribB is negatively affected by FMN and by the FMN analog roseoflavin mononucleotide, which is synthesized enzymatically from roseoflavin and ATP. Consequently, in addition to flavoenzymes, the E. coli ribB FMN riboswitch constitutes a target for the antibiotic roseoflavin produced by Streptomyces davawensis. FMN riboswitches control genes responsible for biosynthesis and/or transport of riboflavin. The Escherichia coli ribB FMN riboswitch controls expression of the essential gene ribB (RibB; EC 4.1.99.12) and operates at the transcriptional and translational level. Expression of ribB is negatively affected by FMN and by roseoflavin mononucleotide (RoFMN), a derivative of the antibiotic roseoflavin produced by Streptomyces davawensis.
| Original language | English |
|---|---|
| Pages (from-to) | 3230-3242 |
| Number of pages | 13 |
| Journal | FEBS Journal |
| Volume | 282 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - 2015 Aug 1 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Cell Biology
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The ribB FMN riboswitch from Escherichia coli operates at the transcriptional and translational level and regulates riboflavin biosynthesis. / Pedrolli, Danielle; Langer, Simone; Hobl, Birgit; Schwarz, Julia; Hashimoto, Masayuki; Mack, Matthias.
In: FEBS Journal, Vol. 282, No. 16, 01.08.2015, p. 3230-3242.Research output: Contribution to journal › Article
TY - JOUR
T1 - The ribB FMN riboswitch from Escherichia coli operates at the transcriptional and translational level and regulates riboflavin biosynthesis
AU - Pedrolli, Danielle
AU - Langer, Simone
AU - Hobl, Birgit
AU - Schwarz, Julia
AU - Hashimoto, Masayuki
AU - Mack, Matthias
PY - 2015/8/1
Y1 - 2015/8/1
N2 - FMN riboswitches are genetic elements that, in many bacteria, control genes responsible for biosynthesis and/or transport of riboflavin (vitamin B2). We report that the Escherichia coli ribB FMN riboswitch controls expression of the essential gene ribB coding for the riboflavin biosynthetic enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase (RibB; EC 4.1.99.12). Our data show that the E. coli ribB FMN riboswitch is unusual because it operates at the transcriptional and also at the translational level. Expression of ribB is negatively affected by FMN and by the FMN analog roseoflavin mononucleotide, which is synthesized enzymatically from roseoflavin and ATP. Consequently, in addition to flavoenzymes, the E. coli ribB FMN riboswitch constitutes a target for the antibiotic roseoflavin produced by Streptomyces davawensis. FMN riboswitches control genes responsible for biosynthesis and/or transport of riboflavin. The Escherichia coli ribB FMN riboswitch controls expression of the essential gene ribB (RibB; EC 4.1.99.12) and operates at the transcriptional and translational level. Expression of ribB is negatively affected by FMN and by roseoflavin mononucleotide (RoFMN), a derivative of the antibiotic roseoflavin produced by Streptomyces davawensis.
AB - FMN riboswitches are genetic elements that, in many bacteria, control genes responsible for biosynthesis and/or transport of riboflavin (vitamin B2). We report that the Escherichia coli ribB FMN riboswitch controls expression of the essential gene ribB coding for the riboflavin biosynthetic enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase (RibB; EC 4.1.99.12). Our data show that the E. coli ribB FMN riboswitch is unusual because it operates at the transcriptional and also at the translational level. Expression of ribB is negatively affected by FMN and by the FMN analog roseoflavin mononucleotide, which is synthesized enzymatically from roseoflavin and ATP. Consequently, in addition to flavoenzymes, the E. coli ribB FMN riboswitch constitutes a target for the antibiotic roseoflavin produced by Streptomyces davawensis. FMN riboswitches control genes responsible for biosynthesis and/or transport of riboflavin. The Escherichia coli ribB FMN riboswitch controls expression of the essential gene ribB (RibB; EC 4.1.99.12) and operates at the transcriptional and translational level. Expression of ribB is negatively affected by FMN and by roseoflavin mononucleotide (RoFMN), a derivative of the antibiotic roseoflavin produced by Streptomyces davawensis.
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U2 - 10.1111/febs.13226
DO - 10.1111/febs.13226
M3 - Article
VL - 282
SP - 3230
EP - 3242
JO - FEBS Journal
JF - FEBS Journal
SN - 1742-464X
IS - 16
ER -