Abstract
FMN riboswitches are genetic elements that, in many bacteria, control genes responsible for biosynthesis and/or transport of riboflavin (vitamin B2). We report that the Escherichia coli ribB FMN riboswitch controls expression of the essential gene ribB coding for the riboflavin biosynthetic enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase (RibB; EC 4.1.99.12). Our data show that the E. coli ribB FMN riboswitch is unusual because it operates at the transcriptional and also at the translational level. Expression of ribB is negatively affected by FMN and by the FMN analog roseoflavin mononucleotide, which is synthesized enzymatically from roseoflavin and ATP. Consequently, in addition to flavoenzymes, the E. coli ribB FMN riboswitch constitutes a target for the antibiotic roseoflavin produced by Streptomyces davawensis. FMN riboswitches control genes responsible for biosynthesis and/or transport of riboflavin. The Escherichia coli ribB FMN riboswitch controls expression of the essential gene ribB (RibB; EC 4.1.99.12) and operates at the transcriptional and translational level. Expression of ribB is negatively affected by FMN and by roseoflavin mononucleotide (RoFMN), a derivative of the antibiotic roseoflavin produced by Streptomyces davawensis.
Original language | English |
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Pages (from-to) | 3230-3242 |
Number of pages | 13 |
Journal | FEBS Journal |
Volume | 282 |
Issue number | 16 |
DOIs | |
Publication status | Published - 2015 Aug 1 |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Cell Biology