The ribB FMN riboswitch from Escherichia coli operates at the transcriptional and translational level and regulates riboflavin biosynthesis

Danielle Pedrolli, Simone Langer, Birgit Hobl, Julia Schwarz, Masayuki Hashimoto, Matthias Mack

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

FMN riboswitches are genetic elements that, in many bacteria, control genes responsible for biosynthesis and/or transport of riboflavin (vitamin B2). We report that the Escherichia coli ribB FMN riboswitch controls expression of the essential gene ribB coding for the riboflavin biosynthetic enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase (RibB; EC 4.1.99.12). Our data show that the E. coli ribB FMN riboswitch is unusual because it operates at the transcriptional and also at the translational level. Expression of ribB is negatively affected by FMN and by the FMN analog roseoflavin mononucleotide, which is synthesized enzymatically from roseoflavin and ATP. Consequently, in addition to flavoenzymes, the E. coli ribB FMN riboswitch constitutes a target for the antibiotic roseoflavin produced by Streptomyces davawensis. FMN riboswitches control genes responsible for biosynthesis and/or transport of riboflavin. The Escherichia coli ribB FMN riboswitch controls expression of the essential gene ribB (RibB; EC 4.1.99.12) and operates at the transcriptional and translational level. Expression of ribB is negatively affected by FMN and by roseoflavin mononucleotide (RoFMN), a derivative of the antibiotic roseoflavin produced by Streptomyces davawensis.

Original languageEnglish
Pages (from-to)3230-3242
Number of pages13
JournalFEBS Journal
Volume282
Issue number16
DOIs
Publication statusPublished - 2015 Aug 1

Fingerprint

Riboswitch
Flavin Mononucleotide
Riboflavin
Biosynthesis
Escherichia coli
Genes
Essential Genes
Streptomyces
Anti-Bacterial Agents
Bacteria
Adenosine Triphosphate

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Pedrolli, Danielle ; Langer, Simone ; Hobl, Birgit ; Schwarz, Julia ; Hashimoto, Masayuki ; Mack, Matthias. / The ribB FMN riboswitch from Escherichia coli operates at the transcriptional and translational level and regulates riboflavin biosynthesis. In: FEBS Journal. 2015 ; Vol. 282, No. 16. pp. 3230-3242.
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The ribB FMN riboswitch from Escherichia coli operates at the transcriptional and translational level and regulates riboflavin biosynthesis. / Pedrolli, Danielle; Langer, Simone; Hobl, Birgit; Schwarz, Julia; Hashimoto, Masayuki; Mack, Matthias.

In: FEBS Journal, Vol. 282, No. 16, 01.08.2015, p. 3230-3242.

Research output: Contribution to journalArticle

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AU - Pedrolli, Danielle

AU - Langer, Simone

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AU - Mack, Matthias

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