To assess the relative effect of interleukin (IL)-2- and IL-4-dependent immune responses on herpes simplex virus (HSV)-1 infection, naive, vaccinated, and mock-vaccinated IL-2(0/0) and IL-4(0/0) knockout mice were challenged ocularly with HSV-1. Naive IL-2(0/0) mice were significantly more susceptible to lethal infection than IL-4(0/0) or parental BALB/c mice. Vaccinated, IL-2(0/0), IL-4(0/0), and BALB/c mice induced similar neutralizing antibody titers and were completely protected against HSV-1- induced death and corneal scarring. Vaccinated and mock-vaccinated IL-2(0/0) mice had significantly higher HSV-1 titers in their eyes than BALB/c mice, while vaccinated and mock-vaccinated IL-4(0/0) mice had significantly lower HSV-1 titers in their eyes than BALB/c mice. Recombinant (r) IL-2 treatment of the IL-2(0/0) mice significantly reduced ocular HSV-1 replications, but rIL-4 treatment of IL-4(0/0) mice significantly increased ocular HSV-1 replications. Th1 (IL-2) cytokine responses may help protect mice against ocular HSV-1 challenge and reduce ocular HSV-1 replication.
All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Infectious Diseases